This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kim, Y. Articles by Matin, A. Search for Related Content PubMed PubMed Citation Articles by Kim, Y. Articles by Matin, A.

 Previous Article  |  Next Article 

J. Bacteriol., Apr 1995, 1850-1859, Vol 177, No. 7
Copyright © 1995, American Society for Microbiology

A carbon starvation survival gene of Pseudomonas putida is regulated by sigma 54

Y Kim, LS Watrud and A Matin
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402.

By using mini-Tn5 transposon mutagenesis, two mutants of Pseudomonas putida ATCC 12633 were isolated which showed a marked increase in their sensitivity to carbon starvation; these mutants are presumably affected in the Pex type of proteins that P. putida induces upon carbon starvation (M. Givskov, L. Eberl, and S. Molin, J. Bacteriol. 176:4816- 4824, 1994). The affected genes in our mutants were induced about threefold upon carbon starvation. The promoter region of the starvation gene in the mutant MK107 possessed a strong sigma 54-type-promoter sequence, and deletion analysis suggested that this was the major promoter regulating expression; this was confirmed by transcript mapping in rpoN+ and rpoN mutant backgrounds. The deletion analysis implicated a sequence upstream of the sigma 54 promoter, as well as a region downstream of the transcription start site, in the functioning of the promoter. Two sigma 70-type Pribnow boxes were also detected in the promoter region, but their transcriptional activity in the wild type was very weak. However, in a sigma 54-deficient background, these promoters became stronger. The mechanism and possible physiological role of this phenomenon and the possibility that the sequence upstream of the sigma 54 promoter may have a role in carbon sensing are discussed.

This article has been cited by other articles:

• King, G. M. (2003). Uptake of Carbon Monoxide and Hydrogen at Environmentally Relevant Concentrations by Mycobacteria{dagger}. Appl. Environ. Microbiol. 69: 7266-7272 [Abstract] [Full Text]  
• Eberl, L., Ammendola, A., Rothballer, M. H., Givskov, M., Sternberg, C., Kilstrup, M., Schleifer, K.-H., Molin, S. (2000). Inactivation of gltB Abolishes Expression of the Assimilatory Nitrate Reductase Gene (nasB) in Pseudomonas putida KT2442. J. Bacteriol. 182: 3368-3376 [Abstract] [Full Text]  
• Park, C. H., Keyhan, M., Wielinga, B., Fendorf, S., Matin, A. (2000). Purification to Homogeneity and Characterization of a Novel Pseudomonas putida Chromate Reductase. Appl. Environ. Microbiol. 66: 1788-1795 [Abstract] [Full Text]  
• Mason, C. A., Dünner, J., Indra, P., Colangelo, T. (1999). Heat-Induced Expression and Chemically Induced Expression of the Escherichia coli Stress Protein HtpG Are Affected by the Growth Environment. Appl. Environ. Microbiol. 65: 3433-3440 [Abstract] [Full Text]  
• Suh, S.-J., Silo-Suh, L., Woods, D. E., Hassett, D. J., West, S. E. H., Ohman, D. E. (1999). Effect of rpoS Mutation on the Stress Response and Expression of Virulence Factors in Pseudomonas aeruginosa. J. Bacteriol. 181: 3890-3897 [Abstract] [Full Text]  
• Ramos-González, M. I., Molin, S. (1998). Cloning, Sequencing, and Phenotypic Characterization of the rpoS Gene from Pseudomonas putida KT2440. J. Bacteriol. 180: 3421-3431 [Abstract] [Full Text]