This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by de la Cruz, J. Articles by Llobell, A. Search for Related Content PubMed PubMed Citation Articles by de la Cruz, J. Articles by Llobell, A.

 Previous Article  |  Next Article 

J. Bacteriol., Apr 1995, 1864-1871, Vol 177, No. 7
Copyright © 1995, American Society for Microbiology

Purification and characterization of an endo-beta-1,6-glucanase from Trichoderma harzianum that is related to its mycoparasitism

J de la Cruz, JA Pintor-Toro, T Benitez and A Llobell
Instituto de Bioquimica Vegetal y Fotosintesis, Facultad de Biologia, Universidad de Sevilla, Spain.

The enzymes from Trichoderma species that degrade fungal cell walls have been suggested to play an important role in mycoparasitic action against fungal plant pathogens. The mycoparasite Trichoderma harzianum produces at least two extracellular beta-1,6-glucanases, among other hydrolases, when it is grown on chitin as the sole carbon source. One of these extracellular enzymes was purified to homogeneity after adsorption to its substrate, pustulan, chromatofocusing, and, finally, gel filtration. The apparent molecular mass was 43,000, and the isoelectric point was 5.8. The first 15 amino acids from the N terminus of the purified protein have been sequenced. The enzyme was specific for beta-1,6 linkages and showed an endolytic mode of action on pustulan. Further characterization indicated that the enzyme by itself releases soluble sugars and produces hydrolytic halli on yeast cell walls. When combined with other T. harzianum cell wall-degrading enzymes such as beta-1,3-glucanases and chitinases, it hydrolyzes filamentous fungal cell walls. The enzyme acts cooperatively with the latter enzymes, inhibiting the growth of the fungi tested. Antibodies against the purified protein also indicated that the two identified beta-1,6-glucanases are not immunologically related and are probably encoded by two different genes.

This article has been cited by other articles:

• Fortwendel, J. R., Fuller, K. K., Stephens, T. J., Bacon, W. C., Askew, D. S., Rhodes, J. C. (2008). Aspergillus fumigatus RasA Regulates Asexual Development and Cell Wall Integrity. Eukaryot Cell 7: 1530-1539 [Abstract] [Full Text]  
• Djonovic, S., Pozo, M. J., Kenerley, C. M. (2006). Tvbgn3, a {beta}-1,6-Glucanase from the Biocontrol Fungus Trichoderma virens, Is Involved in Mycoparasitism and Control of Pythium ultimum. Appl. Environ. Microbiol. 72: 7661-7670 [Abstract] [Full Text]  
• Palma, A. S., Feizi, T., Zhang, Y., Stoll, M. S., Lawson, A. M., Diaz-Rodriguez, E., Campanero-Rhodes, M. A., Costa, J., Gordon, S., Brown, G. D., Chai, W. (2006). Ligands for the beta-Glucan Receptor, Dectin-1, Assigned Using "Designer" Microarrays of Oligosaccharide Probes (Neoglycolipids) Generated from Glucan Polysaccharides. J. Biol. Chem. 281: 5771-5779 [Abstract] [Full Text]  
• Lutz, M. P., Feichtinger, G., Defago, G., Duffy, B. (2003). Mycotoxigenic Fusarium and Deoxynivalenol Production Repress Chitinase Gene Expression in the Biocontrol Agent Trichoderma atroviride P1. Appl. Environ. Microbiol. 69: 3077-3084 [Abstract] [Full Text]  
• Moy, M., Li, H. M., Sullivan, R., White, J. F. Jr., Belanger, F. C. (2002). Endophytic Fungal beta -1,6-Glucanase Expression in the Infected Host Grass. Plant Physiol. 130: 1298-1308 [Abstract] [Full Text]  
• Delgado-Jarana, J., Rincon, A. M., Benitez, T. (2002). Aspartyl protease from Trichoderma harzianum CECT 2413: cloning and characterization. Microbiology 148: 1305-1315 [Abstract] [Full Text]  
• Ait-Lahsen, H., Soler, A., Rey, M., de la Cruz, J., Monte, E., Llobell, A. (2001). An Antifungal Exo-alpha -1,3-Glucanase (AGN13.1) from the Biocontrol Fungus Trichoderma harzianum. Appl. Environ. Microbiol. 67: 5833-5839 [Abstract] [Full Text]  
• Pavia, J., Aguado, C., Mormeneo, S., Sentandreu, R. (2001). Secretion, interaction and assembly of two O-glycosylated cell wall antigens from Candida albicans. Microbiology 147: 1983-1991 [Abstract] [Full Text]  
• Ram, A. F. J., Kapteyn, J. C., Montijn, R. C., Caro, L. H. P., Douwes, J. E., Baginsky, W., Mazur, P., van den Ende, H., Klis, F. M. (1998). Loss of the Plasma Membrane-Bound Protein Gas1p in Saccharomyces cerevisiae Results in the Release of beta 1,3-Glucan into the Medium and Induces a Compensation Mechanism To Ensure Cell Wall Integrity. J. Bacteriol. 180: 1418-1424 [Abstract] [Full Text]