This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lang, H. P. Articles by Hunter, C. N. Search for Related Content PubMed PubMed Citation Articles by Lang, H. P. Articles by Hunter, C. N.

 Previous Article  |  Next Article 

J. Bacteriol., Apr 1995, 2064-2073, Vol 177, No. 8
Copyright © 1995, American Society for Microbiology

Complete DNA sequence, specific Tn5 insertion map, and gene assignment of the carotenoid biosynthesis pathway of Rhodobacter sphaeroides

HP Lang, RJ Cogdell, S Takaichi and CN Hunter
Department of Molecular Biology and Biotechnology, Robert Hill Institute for Photosynthesis and Krebs Institute, University of Sheffield, United Kingdom.

The carotenoid biosynthesis genes form a cluster within the genome of Rhodobacter sphaeroides, lying in the middle of a larger cluster and 45 kb in length, which contains genes for bacteriochlorophyll biosynthesis and for the reaction center and light-harvesting apoproteins. The positions and approximate limits of the carotenoid genes were determined previously by localized transposon Tn5 mutagenesis and by comparison with the closely related Rhodobacter capsulatus carotenoid gene cluster. In this report, analysis of the DNA and deduced amino acid sequences of the carotenoid genes in R. sphaeroides are presented. Twenty-five Tn5 insertion mutants were used to produce a base-specific Tn5 insertion map of this region, and carotenoid gene assignment was supported by spectroscopic, ultrastructural, and high-pressure liquid chromatography analyses of these mutants. A region in the 3' end of crtD which affects bacteriochlorophyll biosynthesis was discovered, and CrtA was found to possess a proline-rich C-terminal region containing a repeated (Ala-Pro)n motif. CrtF also showed a high degree of sequence conservation with eukaryotic O-methyltransferases. This study provides gene sequences and assignments based upon a comprehensive structural, spectroscopic, and biochemical analysis of a range of carotenoid biosynthetic mutants; in each mutation, the point of Tn5 insertion is determined accurate to 1 bp on the gene cluster.

This article has been cited by other articles:

• Hendrischk, A.-K., Braatsch, S., Glaeser, J., Klug, G. (2007). The phrA gene of Rhodobacter sphaeroides encodes a photolyase and is regulated by singlet oxygen and peroxide in a {sigma}E-dependent manner. Microbiology 153: 1842-1851 [Abstract] [Full Text]  
• Glaeser, J., Klug, G. (2005). Photo-oxidative stress in Rhodobacter sphaeroides: protective role of carotenoids and expression of selected genes. Microbiology 151: 1927-1938 [Abstract] [Full Text]  
• Anthony, J. R., Warczak, K. L., Donohue, T. J. (2005). A transcriptional response to singlet oxygen, a toxic byproduct of photosynthesis. Proc. Natl. Acad. Sci. USA 102: 6502-6507 [Abstract] [Full Text]  
• Mohamed, H. E., Vermaas, W. (2004). Slr1293 in Synechocystis sp. Strain PCC 6803 Is the C-3',4' Desaturase (CrtD) Involved in Myxoxanthophyll Biosynthesis. J. Bacteriol. 186: 5621-5628 [Abstract] [Full Text]  
• Kovacs, A. T., Rakhely, G., Kovacs, K. L. (2003). Genes Involved in the Biosynthesis of Photosynthetic Pigments in the Purple Sulfur Photosynthetic Bacterium Thiocapsa roseopersicina. Appl. Environ. Microbiol. 69: 3093-3102 [Abstract] [Full Text]  
• Harada, J., Nagashima, K. V. P., Takaichi, S., Misawa, N., Matsuura, K., Shimada, K. (2001). Phytoene Desaturase, CrtI, of the Purple Photosynthetic Bacterium, Rubrivivax gelatinosus, Produces both Neurosporene and Lycopene. Plant Cell Physiol 42: 1112-1118 [Abstract] [Full Text]  
• Choudhary, M., Kaplan, S. (2000). DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1T. Nucleic Acids Res 28: 862-867 [Abstract] [Full Text]  
• Baker, S. C., Ferguson, S. J., Ludwig, B., Page, M. D., Richter, O.-M. H., van Spanning, R. J. M. (1998). Molecular Genetics of the Genus Paracoccus: Metabolically Versatile Bacteria with Bioenergetic Flexibility. Microbiol. Mol. Biol. Rev. 62: 1046-1078 [Abstract] [Full Text]  
• Zeilstra-Ryalls, J., Gomelsky, M., Eraso, J. M., Yeliseev, A., O'Gara, J., Kaplan, S. (1998). Control of Photosystem Formation in Rhodobacter sphaeroides. J. Bacteriol. 180: 2801-2809 [Full Text]  
• Donohue, T. J. (1997). Eubacterial signal transduction by ligands of the mammalian peripheral benzodiazepine receptor complex. Proc. Natl. Acad. Sci. USA 94: 4821-4822 [Full Text]  
• Ouchane, S., Picaud, M., Vernotte, C., Reiss-Husson, F., Astier, C. (1997). Pleiotropic Effects of puf Interposon Mutagenesis on Carotenoid Biosynthesis in Rubrivivax gelatinosus. A NEW GENE ORGANIZATION IN PURPLE BACTERIA. J. Biol. Chem. 272: 1670-1676 [Abstract] [Full Text]  
• Yeliseev, A. A., Kaplan, S. (1995). A Sensory Transducer Homologous to the Mammalian Peripheral-type Benzodiazepine Receptor Regulates Photosynthetic Membrane Complex Formation in Rhodobacter sphaeroides 2.4.1. J. Biol. Chem. 270: 21167-21175 [Abstract] [Full Text]