J. Bacteriol., 05 1996, 2757-2766, Vol 178, No. 10
Copyright © 1996, American Society for Microbiology

Bradyrhizobium (Arachis) sp. strain NC92 contains two nodD genes involved in the repression of nodA and a nolA gene required for the efficient nodulation of host plants

WK Gillette and GH Elkan
Department of Microbiology, North Carolina State University, Raleigh 27695-7615, USA.

The common nodulation locus and closely linked nodulation genes of Bradyrhizobium (Arachis) sp. strain NC92 have been isolated on an 11.0- kb EcoRI restriction fragment. The nucleotide sequence of a 7.0-kb EcoRV-EcoRI subclone was determined and found to contain open reading frames (ORFs) homologous to the nodA, nodB, nodD1, nodD2, and nolA genes of Bradyrhizobium japonicum and Bradyrhizobium elkanii. Nodulation assays of nodD1, nodD2, or nolA deletion mutants on the host plants Macroptilium atropurpureum (siratro) and Vigna unguiculata (cowpea) indicate that nolA is required for efficient nodulation, as nolA mutants exhibit a 6-day nodulation delay and reduced nodule numbers. The nolA phenotype was complemented by providing the nolA ORF in trans, indicating that the phenotype is due to the lack of the nolA ORF. nodD1 mutants displayed a 2-day nodulation delay, whereas nodD2 strains were indistinguishable from the wild type. Translational nodA- lacZ, nodD1-lacZ, nodD2-lacZ, and nolA-lacZ fusions were created. Expression of the nodA-lacZ fusion was induced by the addition of peanut, cowpea, and siratro seed exudates and by the addition of the isoflavonoids genistein and daidzein. In a nodD1 or nodD2 background, basal expression of the nodA-lacZ fusion increased two- to threefold. The level of expression of the nodD2-lacZ and nolA-lacZ fusions was low in the wild type but increased in nodD1, nodD2, and nodD1 nodD2 backgrounds independently of the addition of the inducer genistein. nolA was required for increased expression of the nodD2-lacZ fusion. These data suggest that a common factor is involved in the regulation of nodD2 and nolA, and they are also consistent with a model of nod gene expression in Bradyrhizobium (Arachis) sp. strain NC92 in which negative regulation is mediated by the products of the nodD1 and nodD2 genes.

This article has been cited by other articles:

• Stepkowski, T., Hughes, C. E., Law, I. J., Markiewicz, L., Gurda, D., Chlebicka, A., Moulin, L. (2007). Diversification of Lupine Bradyrhizobium Strains: Evidence from Nodulation Gene Trees. Appl. Environ. Microbiol. 73: 3254-3264 [Abstract] [Full Text]  
• Loh, J., Carlson, R. W., York, W. S., Stacey, G. (2002). Bradyoxetin, a unique chemical signal involved in symbiotic gene regulation. Proc. Natl. Acad. Sci. USA 99: 14446-14451 [Abstract] [Full Text]  
• Loh, J., Stacey, G. (2002). Nodulation Gene Regulation in Bradyrhizobium japonicum: a Unique Integration of Global Regulatory Circuits. Appl. Environ. Microbiol. 69: 10-17 [Full Text]  
• Loh, J., Stacey, M. G., Sadowsky, M. J., Stacey, G. (1999). The Bradyrhizobium japonicum nolA Gene Encodes Three Functionally Distinct Proteins. J. Bacteriol. 181: 1544-1554 [Abstract] [Full Text]