This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mondigler, M. Articles by Ehrmann, M. Search for Related Content PubMed PubMed Citation Articles by Mondigler, M. Articles by Ehrmann, M.

 Previous Article  |  Next Article 

J. Bacteriol., May 1996, 2986-2988, Vol 178, No. 10
Copyright © 1996, American Society for Microbiology

Site-specific proteolysis of the Escherichia coli SecA protein in vivo

M Mondigler and M Ehrmann
Fakultat fur Biologie, Universitat Konstanz, Germany.

A seven-amino-acid cleavage site specific for tobacco etch virus (TEV) protease was introduced into SecA at two separate positions after amino acids 195 and 252. Chromosomal wild-type secA was replaced by these secA constructs. Simultaneous expression of TEV protease led to cleavage of both SecA derivatives. In the functional SecA dimer, proteolysis directly indicated surface exposure of the TEV protease cleavage sites. Cleavage of SecA near residue 195 generated an unstable proteolysis product and a secretion defect, suggesting that this approach could be used to inactivate essential proteins in vivo.

This article has been cited by other articles:

• Rotem, S., Katz, C., Benyamini, H., Lebendiker, M., Veprintsev, D., Rudiger, S., Danieli, T., Friedler, A. (2008). The Structure and Interactions of the Proline-rich Domain of ASPP2. J. Biol. Chem. 283: 18990-18999 [Abstract] [Full Text]  
• Henrichs, T., Mikhaleva, N., Conz, C., Deuerling, E., Boyd, D., Zelazny, A., Bibi, E., Ban, N., Ehrmann, M. (2005). Target-directed proteolysis at the ribosome. Proc. Natl. Acad. Sci. USA 102: 4246-4251 [Abstract] [Full Text]  
• Ehrmann, M., Bolek, P., Mondigler, M., Boyd, D., Lange, R. (1997). TnTIN and TnTAP: Mini-transposons for site-specific proteolysis vivo. Proc. Natl. Acad. Sci. USA 94: 13111-13115 [Abstract] [Full Text]  
• Faber, K. N., Kram, A. M., Ehrmann, M., Veenhuis, M. (2001). A Novel Method to Determine the Topology of Peroxisomal Membrane Proteins in Vivo Using the Tobacco Etch Virus Protease. J. Biol. Chem. 276: 36501-36507 [Abstract] [Full Text]  
• Gao, X.-D., Dean, N. (2000). Distinct Protein Domains of the Yeast Golgi GDP-mannose Transporter Mediate Oligomer Assembly and Export from the Endoplasmic Reticulum. J. Biol. Chem. 275: 17718-17727 [Abstract] [Full Text]