This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cunningham, T. S.
Right arrow Articles by Cooper, T. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cunningham, T. S.
Right arrow Articles by Cooper, T. G.

 Previous Article  |  Next Article 

J. Bacteriol., Jun 1996, 3470-3479, Vol 178, No. 12
Copyright © 1996, American Society for Microbiology

G1n3p is capable of binding to UAS(NTR) elements and activating transcription in Saccharomyces cerevisiae

TS Cunningham, VV Svetlov, R Rai, W Smart and TG Cooper
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163, USA.

When readily used nitrogen sources are available, the expression of genes encoding proteins needed to transport and metabolize poorly used nitrogen sources is repressed to low levels; this physiological response has been designated nitrogen catabolite repression (NCR). The cis-acting upstream activation sequence (UAS) element UAS(NTR) mediates Gln3p-dependent, NCR-sensitive transcription and consists of two separated dodecanucleotides, each containing the core sequence GATAA. Gln3p, produced in Escherichia coli and hence free of all other yeast proteins, specifically binds to wild-type UAS(NTR) sequences and DNA fragments derived from a variety of NCR-sensitive promoters (GDH2, CAR11 DAL3, PUT1, UGA4, and GLN1). A LexA-Gln3 fusion protein supported transcriptional activation when bound to one or more LexAp binding sites upstream of a minimal CYC1-derived promoter devoid of UAS elements. LexAp-Gln3p activation of transcription was largely independent of the nitrogen source used for growth. These data argue that Gln3p is capable of direct UAS(NTR) binding and participates in transcriptional activation of NCR-sensitive genes.


This article has been cited by other articles:

  • Georis, I., Feller, A., Tate, J. J., Cooper, T. G., Dubois, E. (2009). Nitrogen Catabolite Repression-Sensitive Transcription as a Readout of Tor Pathway Regulation: The Genetic Background, Reporter Gene and GATA Factor Assayed Determine the Outcomes. Genetics 181: 861-874 [Abstract] [Full Text]  
  • Godard, P., Urrestarazu, A., Vissers, S., Kontos, K., Bontempi, G., van Helden, J., Andre, B. (2007). Effect of 21 Different Nitrogen Sources on Global Gene Expression in the Yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 27: 3065-3086 [Abstract] [Full Text]  
  • Powers, R. W. III, Kaeberlein, M., Caldwell, S. D., Kennedy, B. K., Fields, S. (2006). Extension of chronological life span in yeast by decreased TOR pathway signaling. Genes Dev. 20: 174-184 [Abstract] [Full Text]  
  • Das, M., Bhat, P. J. (2005). Disruption of MRG19 results in altered nitrogen metabolic status and defective pseudohyphal development in Saccharomyces cerevisiae. Microbiology 151: 91-98 [Abstract] [Full Text]  
  • Chen, G., Hata, N., Zhang, M. Q. (2004). Transcription factor binding element detection using functional clustering of mutant expression data. Nucleic Acids Res 32: 2362-2371 [Abstract] [Full Text]  
  • Rai, R., Tate, J. J., Cooper, T. G. (2003). Ure2, a Prion Precursor with Homology to Glutathione S-Transferase, Protects Saccharomyces cerevisiae Cells from Heavy Metal Ion and Oxidant Toxicity. J. Biol. Chem. 278: 12826-12833 [Abstract] [Full Text]  
  • Distler, M., Kulkarni, A., Rai, R., Cooper, T. G. (2001). Green Fluorescent Protein-Dal80p Illuminates up to 16 Distinct Foci That Colocalize with and Exhibit the Same Behavior as Chromosomal DNA Proceeding through the Cell Cycle of Saccharomyces cerevisiae. J. Bacteriol. 183: 4636-4642 [Abstract] [Full Text]  
  • Nakagawa, Y., Sugioka, S., Kaneko, Y., Harashima, S. (2001). O2R, a Novel Regulatory Element Mediating Rox1p-Independent O2 and Unsaturated Fatty Acid Repression of OLE1 in Saccharomyces cerevisiae. J. Bacteriol. 183: 745-751 [Abstract] [Full Text]  
  • Cunningham, T. S., Rai, R., Cooper, T. G. (2000). The Level of DAL80 Expression Down-Regulates GATA Factor-Mediated Transcription in Saccharomyces cerevisiae. J. Bacteriol. 182: 6584-6591 [Abstract] [Full Text]  
  • Cunningham, T. S., Andhare, R., Cooper, T. G. (2000). Nitrogen Catabolite Repression of DAL80 Expression Depends on the Relative Levels of Gat1p and Ure2p Production in Saccharomyces cerevisiae. J. Biol. Chem. 275: 14408-14414 [Abstract] [Full Text]  
  • Scott, S., Dorrington, R., Svetlov, V., Beeser, A. E., Distler, M., Cooper, T. G. (2000). Functional Domain Mapping and Subcellular Distribution of Dal82p in Saccharomyces cerevisiae. J. Biol. Chem. 275: 7198-7204 [Abstract] [Full Text]  
  • Rai, R., Daugherty, J. R., Cunningham, T. S., Cooper, T. G. (1999). Overlapping Positive and Negative GATA Factor Binding Sites Mediate Inducible DAL7 Gene Expression in Saccharomyces cerevisiae. J. Biol. Chem. 274: 28026-28034 [Abstract] [Full Text]  
  • Beeser, A. E., Cooper, T. G. (1999). Control of Nitrogen Catabolite Repression Is Not Affected by the tRNAGln-CUU Mutation, Which Results in Constitutive Pseudohyphal Growth of Saccharomyces cerevisiae. J. Bacteriol. 181: 2472-2476 [Abstract] [Full Text]  
  • Svetlov, V. V., Cooper, T. G. (1998). The Saccharomyces cerevisiae GATA Factors Dal80p and Deh1p Can Form Homo- and Heterodimeric Complexes. J. Bacteriol. 180: 5682-5688 [Abstract] [Full Text]  
  • Withee, J. L., Sen, R., Cyert, M. S. (1998). Ion Tolerance of Saccharomyces cerevisiae Lacking the Ca2+/CaM-Dependent Phosphatase (Calcineurin) Is Improved by Mutations in URE2 or PMA1. Genetics 149: 865-878 [Abstract] [Full Text]  
  • van der Merwe, G. K., Cooper, T. G., van Vuuren, H. J. J. (2001). Ammonia Regulates VID30 Expression and Vid30p Function Shifts Nitrogen Metabolism toward Glutamate Formation Especially when Saccharomyces cerevisiae Is Grown in Low Concentrations of Ammonia. J. Biol. Chem. 276: 28659-28666 [Abstract] [Full Text]  
  • Kulkarni, A. A., Abul-Hamd, A. T., Rai, R., El Berry, H., Cooper, T. G. (2001). Gln3p Nuclear Localization and Interaction with Ure2p in Saccharomyces cerevisiae. J. Biol. Chem. 276: 32136-32144 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»