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J. Bacteriol., 06 1996, 3614-3620, Vol 178, No. 12
Y Zhang and JE Cronan Jr
The genes encoding acyl carrier protein and several key fatty acid
biosynthetic enzymes are clustered at min 24 of the Escherichia coli
chromosome. This cluster of genes is not transcribed as a classical operon,
but rather multiple promoters are present and each gene is cotranscribed
with at least one other gene. Transcripts specific for single genes ar also
present. Transcription of acpP, the gene encoding acyl carrier protein, has
been studied in detail. The acpP gene was shown to be transcribed from at
least two different promoters by Northern (RNA) blot, primer extension, and
deletion analyses, and the major promoter was mapped. We tested whether
multiple promoters are necessary to produce acyl carrier protein by use of
a new method whereby a transcriptional terminator was integrated into the
chromosome upstream of the intact acpP gene. By use of this method (called
polar allele duplication), we demonstrate that the promoter located
immediately upstream of the coding sequence is sufficient for synthesis of
this very abundant protein.
Copyright © 1996, American Society for Microbiology
Polar allele duplication for transcriptional analysis of consecutive essential genes: application to a cluster of Escherichia coli fatty acid biosynthetic genes
Departments of Microbiology and Biochemistry, University of Illinois, Urbana 61801, USA.
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