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J. Bacteriol., Jun 1996, 3658-3660, Vol 178, No. 12
Copyright © 1996, American Society for Microbiology

Characterization of the major citrate synthase of Bacillus subtilis

S Jin and AL Sonenshein
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.

The major citrate synthase of Bacillus subtilis (CS-II) was purified to near homogeneity and shown to correspond to the product of the citZ gene. Accumulation of CS-II during exponential growth and stationary phases paralleled expression of the citZ gene. The physical and kinetic properties of CS-II were similar to those of citrate synthase enzymes from Bacillus megaterium and from eukaryotic cells but differed from those of citrate synthases from many gram-negative bacteria.


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