J. Bacteriol., 06 1996, 3661-3663, Vol 178, No. 12
Copyright © 1996, American Society for Microbiology

Genetic analysis of plasmid determinants for microcin J25 production and immunity

JO Solbiati, M Ciaccio, RN Farias and RA Salomon
Departamento de Bioquimica de la Nutricion, Instituto Superior de Investigaciones Biologicas, Tucuman, Argentina.

Microcin J25 (MccJ25) is a small peptide antibiotic produced by an Escherichia coli strain isolated from human feces. The genetic determinants for MccJ25 synthesis and immunity have been cloned from the low-copy-number wild-type plasmid pTUC1OO into the compatible vectors pBR322 and pACYC184. Physical and phenotypical analysis of insertion mutations and complementation tests defined three contiguous genes involved in MccJ25 production which span a region of about 2.2 kb. Immunity to the antibiotic is provided by an additional gene adjacent to the production region.

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