This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hommes, N. G.
Right arrow Articles by Arp, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hommes, N. G.
Right arrow Articles by Arp, D. J.

 Previous Article  |  Next Article 

J. Bacteriol., 07 1996, 3710-3714, Vol 178, No. 13
Copyright © 1996, American Society for Microbiology

Mutagenesis of hydroxylamine oxidoreductase in Nitrosomonas europaea by transformation and recombination

NG Hommes, LA Sayavedra-Soto and DJ Arp
Laboratory for Nitrogen Fixation Research, Oregon State University, Corvallis, Oregon 97331-2902, USA.

Mutagenesis of Nitrosomonas europaea was achieved by electroporation and recombination. To demonstrate this, an aminoglycoside 3'- phosphotransferase (kan) gene was specifically inserted into each of the three gene copies of hao individually. Southern hybridizations and PCR analysis showed the incorporation of the kan gene at the chosen genetic loci. The isolation of mutant strains was achieved in 7 to 14 days when the strains were grown on solid medium. The induced mutations were stable even in the absence of kanamycin-selective pressure for periods of up to 45 days in culture. The mutant strains did not show an observable phenotype different from that of the wild type when grown under the same conditions.


This article has been cited by other articles:

  • Cherif-Zahar, B., Durand, A., Schmidt, I., Hamdaoui, N., Matic, I., Merrick, M., Matassi, G. (2007). Evolution and Functional Characterization of the RH50 Gene from the Ammonia-Oxidizing Bacterium Nitrosomonas europaea. J. Bacteriol. 189: 9090-9100 [Abstract] [Full Text]  
  • Wei, X., Sayavedra-Soto, L. A., Arp, D. J. (2007). Characterization of the ferrioxamine uptake system of Nitrosomonas europaea. Microbiology 153: 3963-3972 [Abstract] [Full Text]  
  • Hommes, N. G., Kurth, E. G., Sayavedra-Soto, L. A., Arp, D. J. (2006). Disruption of sucA, Which Encodes the E1 Subunit of {alpha}-Ketoglutarate Dehydrogenase, Affects the Survival of Nitrosomonas europaea in Stationary Phase. J. Bacteriol. 188: 343-347 [Abstract] [Full Text]  
  • Wei, X., Sayavedra-Soto, L. A., Arp, D. J. (2004). The transcription of the cbb operon in Nitrosomonas europaea. Microbiology 150: 1869-1879 [Abstract] [Full Text]  
  • Hommes, N. G., Sayavedra-Soto, L. A., Arp, D. J. (2003). Chemolithoorganotrophic Growth of Nitrosomonas europaea on Fructose. J. Bacteriol. 185: 6809-6814 [Abstract] [Full Text]  
  • Chain, P., Lamerdin, J., Larimer, F., Regala, W., Lao, V., Land, M., Hauser, L., Hooper, A., Klotz, M., Norton, J., Sayavedra-Soto, L., Arciero, D., Hommes, N., Whittaker, M., Arp, D. (2003). Complete Genome Sequence of the Ammonia-Oxidizing Bacterium and Obligate Chemolithoautotroph Nitrosomonas europaea. J. Bacteriol. 185: 2759-2773 [Abstract] [Full Text]  
  • Beaumont, H. J. E., Hommes, N. G., Sayavedra-Soto, L. A., Arp, D. J., Arciero, D. M., Hooper, A. B., Westerhoff, H. V., van Spanning, R. J. M. (2002). Nitrite Reductase of Nitrosomonas europaea Is Not Essential for Production of Gaseous Nitrogen Oxides and Confers Tolerance to Nitrite. J. Bacteriol. 184: 2557-2560 [Abstract] [Full Text]  
  • Hommes, N. G., Sayavedra-Soto, L. A., Arp, D. J. (2001). Transcript Analysis of Multiple Copies of amo (Encoding Ammonia Monooxygenase) and hao (Encoding Hydroxylamine Oxidoreductase) in Nitrosomonas europaea. J. Bacteriol. 183: 1096-1100 [Abstract] [Full Text]  
  • Hirota, R., Yamagata, A., Kato, J., Kuroda, A., Ikeda, T., Takiguchi, N., Ohtake, H. (2000). Physical Map Location of the Multicopy Genes Coding for Ammonia Monooxygenase and Hydroxylamine Oxidoreductase in the Ammonia-Oxidizing Bacterium Nitrosomonas sp. Strain ENI-11. J. Bacteriol. 182: 825-828 [Abstract] [Full Text]  
  • Yamagata, A., Kato, J., Hirota, R., Kuroda, A., Ikeda, T., Takiguchi, N., Ohtake, H. (1999). Isolation and Characterization of Two Cryptic Plasmids in the Ammonia-Oxidizing Bacterium Nitrosomonas sp. Strain ENI-11. J. Bacteriol. 181: 3375-3381 [Abstract] [Full Text]  
  • Hommes, N. G., Sayavedra-Soto, L. A., Arp, D. J. (1998). Mutagenesis and Expression of amo, Which Codes for Ammonia Monooxygenase in Nitrosomonas europaea. J. Bacteriol. 180: 3353-3359 [Abstract] [Full Text]