FtsZ ring formation in fts mutants

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Addinall, S. G.
	Articles by Lutkenhaus, J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Addinall, S. G.
	Articles by Lutkenhaus, J.

J. Bacteriol., Jul 1996, 3877-3884, Vol 178, No. 13
Copyright © 1996, American Society for Microbiology

FtsZ ring formation in fts mutants

SG Addinall, E Bi and J Lutkenhaus
Department of Microbiology, Molecular Genetics and Immunology, Kansas 66160, USA.

The formation of FtsZ rings (Z rings) in various fts mutants was examined by immunoelectron microscopy and immunofluorescence. In two temperature-sensitive ftsZ mutants which form filaments with smooth morphology, the Z ring was unable to form. In ftsA, ftsI, and ftsQ mutants, which form filaments with an indented morphology, Z rings formed but their contraction was blocked. These results indicate that fully functional ftsA, ftsQ, and ftsI genes are not required for Z-ring formation and are unlikely to have a role in localization of the Z ring. The results also suggest that one function of the Z ring is to localize the activity of other fts gene products.

This article has been cited by other articles:

Jaiswal, R., Panda, D. (2008). Cysteine 155 plays an important role in the assembly of Mycobacterium tuberculosis FtsZ. Protein Sci. 17: 846-854 [Abstract] [Full Text]
Dajkovic, A., Mukherjee, A., Lutkenhaus, J. (2008). Investigation of Regulation of FtsZ Assembly by SulA and Development of a Model for FtsZ Polymerization. J. Bacteriol. 190: 2513-2526 [Abstract] [Full Text]
Vats, P., Rothfield, L. (2007). Duplication and segregation of the actin (MreB) cytoskeleton during the prokaryotic cell cycle. Proc. Natl. Acad. Sci. USA 104: 17795-17800 [Abstract] [Full Text]
Corbin, B. D., Wang, Y., Beuria, T. K., Margolin, W. (2007). Interaction between Cell Division Proteins FtsE and FtsZ. J. Bacteriol. 189: 3026-3035 [Abstract] [Full Text]
Maple, J., Moller, S. G. (2007). Plastid Division: Evolution, Mechanism and Complexity. ANN BOT (LOND) 99: 565-579 [Abstract] [Full Text]
Geissler, B., Shiomi, D., Margolin, W. (2007). The ftsA* gain-of-function allele of Escherichia coli and its effects on the stability and dynamics of the Z ring. Microbiology 153: 814-825 [Abstract] [Full Text]
Thakur, M., Chakraborti, P. K. (2006). GTPase Activity of Mycobacterial FtsZ Is Impaired Due to Its Transphosphorylation by the Eukaryotic-type Ser/Thr Kinase, PknA. J. Biol. Chem. 281: 40107-40113 [Abstract] [Full Text]
Susin, M. F., Baldini, R. L., Gueiros-Filho, F., Gomes, S. L. (2006). GroES/GroEL and DnaK/DnaJ Have Distinct Roles in Stress Responses and during Cell Cycle Progression in Caulobacter crescentus. J. Bacteriol. 188: 8044-8053 [Abstract] [Full Text]
Chapman, E., Farr, G. W., Usaite, R., Furtak, K., Fenton, W. A., Chaudhuri, T. K., Hondorp, E. R., Matthews, R. G., Wolf, S. G., Yates, J. R., Pypaert, M., Horwich, A. L. (2006). Global aggregation of newly translated proteins in an Escherichia coli strain deficient of the chaperonin GroEL. Proc. Natl. Acad. Sci. USA 103: 15800-15805 [Abstract] [Full Text]
Shih, Y.-L., Rothfield, L. (2006). The Bacterial Cytoskeleton. Microbiol. Mol. Biol. Rev. 70: 729-754 [Abstract] [Full Text]
Sakr, S., Thyssen, M., Denis, M., Zhang, C.-C. (2006). Relationship among Several Key Cell Cycle Events in the Developmental Cyanobacterium Anabaena sp. Strain PCC 7120.. J. Bacteriol. 188: 5958-5965 [Abstract] [Full Text]
Jensen, S. O., Thompson, L. S., Harry, E. J. (2005). Cell Division in Bacillus subtilis: FtsZ and FtsA Association Is Z-Ring Independent, and FtsA Is Required for Efficient Midcell Z-Ring Assembly. J. Bacteriol. 187: 6536-6544 [Abstract] [Full Text]
Sontag, C. A., Staley, J. T., Erickson, H. P. (2005). In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB. J. Cell Biol. 169: 233-238 [Abstract] [Full Text]
Addinall, S. G., Small, E., Whitaker, D., Sturrock, S., Donachie, W. D., Khattar, M. M. (2005). New Temperature-Sensitive Alleles of ftsZ in Escherichia coli. J. Bacteriol. 187: 358-365 [Abstract] [Full Text]
Janakiraman, A., Goldberg, M. B. (2004). From the Cover: Evidence for polar positional information independent of cell division and nucleoid occlusion. Proc. Natl. Acad. Sci. USA 101: 835-840 [Abstract] [Full Text]
Di Lallo, G., Fagioli, M., Barionovi, D., Ghelardini, P., Paolozzi, L. (2003). Use of a two-hybrid assay to study the assembly of a complex multicomponent protein machinery: bacterial septosome differentiation. Microbiology 149: 3353-3359 [Abstract] [Full Text]
Scholle, M. D., White, C. A., Kunnimalaiyaan, M., Vary, P. S. (2003). Sequencing and Characterization of pBM400 from Bacillus megaterium QM B1551. Appl. Environ. Microbiol. 69: 6888-6898 [Abstract] [Full Text]
Stricker, J., Erickson, H. P. (2003). In Vivo Characterization of Escherichia coli ftsZ Mutants: Effects on Z-Ring Structure and Function. J. Bacteriol. 185: 4796-4805 [Abstract] [Full Text]
Small, E., Addinall, S. G. (2003). Dynamic FtsZ polymerization is sensitive to the GTP to GDP ratio and can be maintained at steady state using a GTP-regeneration system. Microbiology 149: 2235-2242 [Abstract] [Full Text]
Jankovic, I., Ventura, M., Meylan, V., Rouvet, M., Elli, M., Zink, R. (2003). Contribution of Aggregation-Promoting Factor to Maintenance of Cell Shape in Lactobacillusgasseri 4B2. J. Bacteriol. 185: 3288-3296 [Abstract] [Full Text]
Errington, J., Daniel, R. A., Scheffers, D.-J. (2003). Cytokinesis in Bacteria. Microbiol. Mol. Biol. Rev. 67: 52-65 [Abstract] [Full Text]
Gueiros-Filho, F. J., Losick, R. (2002). A widely conserved bacterial cell division protein that promotes assembly of the tubulin-like protein FtsZ. Genes Dev. 16: 2544-2556 [Abstract] [Full Text]
Johnson, J. E., Lackner, L. L., de Boer, P. A. J. (2002). Targeting of DMinC/MinD and DMinC/DicB Complexes to Septal Rings in Escherichia coli Suggests a Multistep Mechanism for MinC-Mediated Destruction of Nascent FtsZ Rings. J. Bacteriol. 184: 2951-2962 [Abstract] [Full Text]
Hale, C. A., de Boer, P. A. J. (2002). ZipA Is Required for Recruitment of FtsK, FtsQ, FtsL, and FtsN to the Septal Ring in Escherichia coli. J. Bacteriol. 184: 2552-2556 [Abstract] [Full Text]
Mercer, K. L. N., Weiss, D. S. (2002). The Escherichia coli Cell Division Protein FtsW Is Required To Recruit Its Cognate Transpeptidase, FtsI (PBP3), to the Division Site. J. Bacteriol. 184: 904-912 [Abstract] [Full Text]
Fujishima, H., Nishimura, A., Wachi, M., Takagi, H., Hirasawa, T., Teraoka, H., Nishimori, K., Kawabata, T., Nishikawa, K., Nagai, K. (2002). kdsA mutations affect FtsZ-ring formation in Escherichia coli K-12. Microbiology 148: 103-112 [Abstract] [Full Text]
Pichoff, S., Lutkenhaus, J. (2001). Escherichia coli Division Inhibitor MinCD Blocks Septation by Preventing Z-Ring Formation. J. Bacteriol. 183: 6630-6635 [Abstract] [Full Text]
Seto, S., Layh-Schmitt, G., Kenri, T., Miyata, M. (2001). Visualization of the Attachment Organelle and Cytadherence Proteins of Mycoplasma pneumoniae by Immunofluorescence Microscopy. J. Bacteriol. 183: 1621-1630 [Abstract] [Full Text]
Fu, X., Shih, Y.-L., Zhang, Y., Rothfield, L. I. (2001). The MinE ring required for proper placement of the division site is a mobile structure that changes its cellular location during the Escherichia coli division cycle. Proc. Natl. Acad. Sci. USA 10.1073/pnas.031549298v1 [Abstract] [Full Text]
Newman, G., Crooke, E. (2000). DnaA, the Initiator of Escherichia coli Chromosomal Replication, Is Located at the Cell Membrane. J. Bacteriol. 182: 2604-2610 [Abstract] [Full Text]
Lu, C., Reedy, M., Erickson, H. P. (2000). Straight and Curved Conformations of FtsZ Are Regulated by GTP Hydrolysis. J. Bacteriol. 182: 164-170 [Abstract] [Full Text]
Hu, Z., Mukherjee, A., Pichoff, S., Lutkenhaus, J. (1999). The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization. Proc. Natl. Acad. Sci. USA 96: 14819-14824 [Abstract] [Full Text]
Guerrero-Barrera, A. L., de la Garza, M., Mondragón, R., García-Cuéllar, C., Segura-Nieto, M. (1999). Actin-related proteins in Actinobacillus pleuropneumoniae and their interactions with actin-binding proteins. Microbiology 145: 3235-3244 [Abstract] [Full Text]
Maier, S. K., Scherer, S., Loessner, M. J. (1999). Long-Chain Polyphosphate Causes Cell Lysis and Inhibits Bacillus cereus Septum Formation, Which Is Dependent on Divalent Cations. Appl. Environ. Microbiol. 65: 3942-3949 [Abstract] [Full Text]
Pedersen, L. B., Angert, E. R., Setlow, P. (1999). Septal Localization of Penicillin-Binding Protein 1�in Bacillus subtilis. J. Bacteriol. 181: 3201-3211 [Abstract] [Full Text]
Cook, W. R., Rothfield, L. I. (1999). Nucleoid-Independent Identification of Cell Division Sites in Escherichia coli. J. Bacteriol. 181: 1900-1905 [Abstract] [Full Text]
Mukherjee, A., Lutkenhaus, J. (1999). Analysis of FtsZ Assembly by Light Scattering and Determination of the Role of Divalent Metal Cations. J. Bacteriol. 181: 823-832 [Abstract] [Full Text]
Weiss, D. S., Chen, J. C., Ghigo, J.-M., Boyd, D., Beckwith, J. (1999). Localization of FtsI (PBP3) to the Septal Ring Requires Its Membrane Anchor, the Z Ring, FtsA, FtsQ, and FtsL. J. Bacteriol. 181: 508-520 [Abstract] [Full Text]
Chen, J. C., Weiss, D. S., Ghigo, J.-M., Beckwith, J. (1999). Septal Localization of FtsQ, an Essential Cell Division Protein in Escherichia coli. J. Bacteriol. 181: 521-530 [Abstract] [Full Text]
Hale, C. A., de Boer, P. A.�J. (1999). Recruitment of ZipA to the Septal Ring of Escherichia coli Is Dependent on FtsZ and Independent of FtsA. J. Bacteriol. 181: 167-176 [Abstract] [Full Text]
Buddelmeijer, N., Aarsman, M. E.�G., Kolk, A. H.�J., Vicente, M., Nanninga, N. (1998). Localization of Cell Division Protein FtsQ by Immunofluorescence Microscopy in Dividing and Nondividing Cells of Escherichia coli. J. Bacteriol. 180: 6107-6116 [Abstract] [Full Text]
Levin, P. A., Shim, J. J., Grossman, A. D. (1998). Effect of minCD on FtsZ Ring Position and Polar Septation in Bacillus subtilis. J. Bacteriol. 180: 6048-6051 [Abstract] [Full Text]
Marston, A. L., Thomaides, H. B., Edwards, D. H., Sharpe, M. E., Errington, J. (1998). Polar localization of the MinD protein of Bacillus subtilis and its role in selection of the mid-cell division site. Genes Dev. 12: 3419-3430 [Abstract] [Full Text]
Draper, G. C., McLennan, N., Begg, K., Masters, M., Donachie, W. D. (1998). Only the N-Terminal Domain of FtsK Functions in Cell Division. J. Bacteriol. 180: 4621-4627 [Abstract] [Full Text]
Mileykovskaya, E., Sun, Q., Margolin, W., Dowhan, W. (1998). Localization and Function of Early Cell Division Proteins in Filamentous Escherichia coli Cells Lacking Phosphatidylethanolamine. J. Bacteriol. 180: 4252-4257 [Abstract] [Full Text]
Trusca, D., Scott, S., Thompson, C., Bramhill, D. (1998). Bacterial SOS Checkpoint Protein SulA Inhibits Polymerization of Purified FtsZ Cell Division Protein. J. Bacteriol. 180: 3946-3953 [Abstract] [Full Text]
Ukai, H., Matsuzawa, H., Ito, K., Yamada, M., Nishimura, A. (1998). ftsE(Ts) Affects Translocation of K+-Pump Proteins into the Cytoplasmic Membrane of Escherichia coli. J. Bacteriol. 180: 3663-3670 [Abstract] [Full Text]
Pogliano, J., Dong, J. M., De Wulf, P., Furlong, D., Boyd, D., Losick, R., Pogliano, K., Lin, E. C.�C. (1998). Aberrant Cell Division and Random FtsZ Ring Positioning in Escherichia coli cpxA* Mutants. J. Bacteriol. 180: 3486-3490 [Abstract] [Full Text]
Wang, L., Khattar, M. K., Donachie, W. D., Lutkenhaus, J. (1998). FtsI and FtsW Are Localized to the Septum in Escherichia coli. J. Bacteriol. 180: 2810-2816 [Abstract] [Full Text]
Sun, Q., Margolin, W. (1998). FtsZ Dynamics during the Division Cycle of Live Escherichia coli Cells. J. Bacteriol. 180: 2050-2056 [Abstract] [Full Text]
Mukherjee, A., Cao, C., Lutkenhaus, J. (1998). Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia�coli. Proc. Natl. Acad. Sci. USA 95: 2885-2890 [Abstract] [Full Text]
Kelly, A. J., Sackett, M. J., Din, N., Quardokus, E., Brun, Y. V. (1998). Cell cycle-dependent transcriptional and proteolytic regulation of FtsZ in�Caulobacter. Genes Dev. 12: 880-893 [Abstract] [Full Text]
Yu, X.-c., Tran, A. H., Sun, Q., Margolin, W. (1998). Localization of Cell Division Protein FtsK to the Escherichia coli Septum and Identification of a Potential N-Terminal Targeting Domain. J. Bacteriol. 180: 1296-1304 [Abstract] [Full Text]
Begg, K., Nikolaichik, Y., Crossland, N., Donachie, W. D. (1998). Roles of FtsA and FtsZ in Activation of Division Sites. J. Bacteriol. 180: 881-884 [Abstract] [Full Text]
Seto, S., Miyata, M. (1998). Cell Reproduction and Morphological Changes in Mycoplasma capricolum. J. Bacteriol. 180: 256-264 [Abstract] [Full Text]
Lin, D. C.-H., Levin, P. A., Grossman, A. D. (1997). Bipolar localization of a chromosome partition protein in Bacillus�subtilis. Proc. Natl. Acad. Sci. USA 94: 4721-4726 [Abstract] [Full Text]
Pogliano, J., Pogliano, K., Weiss, D.�S., Losick, R., Beckwith, J. (1997). Inactivation of FtsI inhibits constriction of the FtsZ cytokinetic ring and delays the assembly of FtsZ rings at potential�division�sites. Proc. Natl. Acad. Sci. USA 94: 559-564 [Abstract] [Full Text]
Ma, X., Ehrhardt, D.�W., Margolin, W. (1996). Colocalization of cell division proteins FtsZ and FtsA to cytoskeletal structures in living Escherichia coli cells by using green�fluorescent�protein. Proc. Natl. Acad. Sci. USA 93: 12998-13003 [Abstract] [Full Text]
Fu, X., Shih, Y.-L., Zhang, Y., Rothfield, L. I. (2001). The MinE ring required for proper placement of the division site is a mobile structure that changes its cellular location during the Escherichia coli division cycle. Proc. Natl. Acad. Sci. USA 98: 980-985 [Abstract] [Full Text]

Appl. Environ. Microbiol.	Infect. Immun.	Eukaryot. Cell
Mol. Cell. Biol.	J. Virol.	Microbiol. Mol. Biol. Rev.
	ALL ASM JOURNALS