This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kido, M.
Right arrow Articles by Hiraga, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kido, M.
Right arrow Articles by Hiraga, S.

 Previous Article  |  Next Article 

J. Bacteriol., 07 1996, 3917-3925, Vol 178, No. 13
Copyright © 1996, American Society for Microbiology

RNase E polypeptides lacking a carboxyl-terminal half suppress a mukB mutation in Escherichia coli

M Kido, K Yamanaka, T Mitani, H Niki, T Ogura and S Hiraga
Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto, Japan.

We have isolated suppressor mutants that suppress temperature-sensitive colony formation and anucleate cell production of a mukB mutation. A linkage group (smbB) of the suppressor mutations is located in the rne/ams/hmp gene encoding the processing endoribonuclease RNase E. All of the rne (smbB) mutants code for truncated RNase E polypeptides lacking a carboxyl-terminal half. The amount of MukB protein was higher in these rne mutants than that in the rne+ strain. These rne mutants grew nearly normally in the mukB+ genetic background. The copy number of plasmid pBR322 in these rne mutants was lower than that in the rne+ isogenic strain. The results suggest that these rne mutations increase the half-lives of mukB mRNA and RNAI of pBR322, the antisense RNA regulating ColE1-type plasmid replication. We have demonstrated that the wild-type RNase E protein bound to polynucleotide phosphorylase (PNPase) but a truncated RNase E polypeptide lacking the C-terminal half did not. We conclude that the C-terminal half of RNase E is not essential for viability but plays an important role for binding with PNPase. RNase E and PNPase of the multiprotein complex presumably cooperate for effective processing and turnover of specific substrates, such as mRNAs and other RNAs in vivo.


This article has been cited by other articles:

  • Cardenas, P. P., Carrasco, B., Sanchez, H., Deikus, G., Bechhofer, D. H, Alonso, J. C (2009). Bacillus subtilis polynucleotide phosphorylase 3'-to-5' DNase activity is involved in DNA repair. Nucleic Acids Res 37: 4157-4169 [Abstract] [Full Text]  
  • Singh, D., Chang, S.-J., Lin, P.-H., Averina, O. V., Kaberdin, V. R., Lin-Chao, S. (2009). Regulation of ribonuclease E activity by the L4 ribosomal protein of Escherichia coli. Proc. Natl. Acad. Sci. USA 106: 864-869 [Abstract] [Full Text]  
  • Guillier, M., Gottesman, S. (2008). The 5' end of two redundant sRNAs is involved in the regulation of multiple targets, including their own regulator. Nucleic Acids Res 36: 6781-6794 [Abstract] [Full Text]  
  • Shin, E., Go, H., Yeom, J.-H., Won, M., Bae, J., Han, S. H., Han, K., Lee, Y., Ha, N.-C., Moore, C. J., Sohlberg, B., Cohen, S. N., Lee, K. (2008). Identification of Amino Acid Residues in the Catalytic Domain of RNase E Essential for Survival of Escherichia coli: Functional Analysis of DNase I Subdomain. Genetics 179: 1871-1879 [Abstract] [Full Text]  
  • Hankins, J. S., Zappavigna, C., Prud'homme-Genereux, A., Mackie, G. A. (2007). Role of RNA Structure and Susceptibility to RNase E in Regulation of a Cold Shock mRNA, cspA mRNA. J. Bacteriol. 189: 4353-4358 [Abstract] [Full Text]  
  • Avadhani, M., Geyer, R., White, D. C., Shimkets, L. J. (2006). Lysophosphatidylethanolamine Is a Substrate for the Short-Chain Alcohol Dehydrogenase SocA from Myxococcus xanthus. J. Bacteriol. 188: 8543-8550 [Abstract] [Full Text]  
  • Petrushenko, Z. M., Lai, C.-H., Rybenkov, V. V. (2006). Antagonistic Interactions of Kleisins and DNA with Bacterial Condensin MukB. J. Biol. Chem. 281: 34208-34217 [Abstract] [Full Text]  
  • Aiso, T., Yoshida, H., Wada, A., Ohki, R. (2005). Modulation of mRNA Stability Participates in Stationary-Phase-Specific Expression of Ribosome Modulation Factor. J. Bacteriol. 187: 1951-1958 [Abstract] [Full Text]  
  • Case, R. B., Chang, Y.-P., Smith, S. B., Gore, J., Cozzarelli, N. R., Bustamante, C. (2004). The Bacterial Condensin MukBEF Compacts DNA into a Repetitive, Stable Structure. Science 305: 222-227 [Abstract] [Full Text]  
  • Bernstein, J. A., Lin, P.-H., Cohen, S. N., Lin-Chao, S. (2004). Global analysis of Escherichia coli RNA degradosome function using DNA microarrays. Proc. Natl. Acad. Sci. USA 101: 2758-2763 [Abstract] [Full Text]  
  • Zhan, X., Gao, J., Jain, C., Cieslewicz, M. J., Swartz, J. R., Georgiou, G. (2004). Genetic Analysis of Disulfide Isomerization in Escherichia coli: Expression of DsbC Is Modulated by RNase E-Dependent mRNA Processing. J. Bacteriol. 186: 654-660 [Abstract] [Full Text]  
  • Redko, Y., Tock, M. R., Adams, C. J., Kaberdin, V. R., Grasby, J. A., McDowall, K. J. (2003). Determination of the Catalytic Parameters of the N-terminal Half of Escherichia coli Ribonuclease E and the Identification of Critical Functional Groups in RNA Substrates. J. Biol. Chem. 278: 44001-44008 [Abstract] [Full Text]  
  • Carpousis, A. J. (2003). Degradation of targeted mRNAs in Escherichia coli: regulation by a small antisense RNA. Genes Dev. 17: 2351-2355 [Full Text]  
  • Otsuka, Y., Ueno, H., Yonesaki, T. (2003). Escherichia coli Endoribonucleases Involved in Cleavage of Bacteriophage T4 mRNAs. J. Bacteriol. 185: 983-990 [Abstract] [Full Text]  
  • Liou, G.-G., Chang, H.-Y., Lin, C.-S., Lin-Chao, S. (2002). DEAD Box RhlB RNA Helicase Physically Associates with Exoribonuclease PNPase to Degrade Double-stranded RNA Independent of the Degradosome-assembling Region of RNase E. J. Biol. Chem. 277: 41157-41162 [Abstract] [Full Text]  
  • Jager, S., Fuhrmann, O., Heck, C., Hebermehl, M., Schiltz, E., Rauhut, R., Klug, G. (2001). An mRNA degrading complex in Rhodobacter capsulatus. Nucleic Acids Res 29: 4581-4588 [Abstract] [Full Text]  
  • Yamanaka, K., Inouye, M. (2001). Selective mRNA Degradation by Polynucleotide Phosphorylase in Cold Shock Adaptation in Escherichia coli. J. Bacteriol. 183: 2808-2816 [Abstract] [Full Text]  
  • Liou, G.-G., Jane, W.-N., Cohen, S. N., Lin, N.-S., Lin-Chao, S. (2000). RNA degradosomes exist in vivo in Escherichia coli as multicomponent complexes associated with the cytoplasmic membrane via the N-terminal region of ribonuclease E. Proc. Natl. Acad. Sci. USA 10.1073/pnas.011535498v1 [Abstract] [Full Text]  
  • Jiang, X., Diwa, A., Belasco, J. G. (2000). Regions of RNase E Important for 5'-End-Dependent RNA Cleavage and Autoregulated Synthesis. J. Bacteriol. 182: 2468-2475 [Abstract] [Full Text]  
  • Kaberdin, V. R., Miczak, A., Jakobsen, J. S., Lin-Chao, S., McDowall, K. J., von Gabain, A. (1998). The endoribonucleolytic N-terminal half of Escherichia coli RNase E is evolutionarily conserved in Synechocystis sp. and other bacteria but not the C-terminal half, which is sufficient for degradosome assembly. Proc. Natl. Acad. Sci. USA 95: 11637-11642 [Abstract] [Full Text]  
  • Vanzo, N. F., Li, Y. S., Py, B., Blum, E., Higgins, C. F., Raynal, L. C., Krisch, H. M., Carpousis, A. J. (1998). Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome. Genes Dev. 12: 2770-2781 [Abstract] [Full Text]  
  • Liou, G.-G., Jane, W.-N., Cohen, S. N., Lin, N.-S., Lin-Chao, S. (2001). RNA degradosomes exist in vivo in Escherichia coli as multicomponent complexes associated with the cytoplasmic membrane via the N-terminal region of ribonuclease E. Proc. Natl. Acad. Sci. USA 98: 63-68 [Abstract] [Full Text]  
  • Sawitzke, J. A., Austin, S. (2000). Suppression of chromosome segregation defects of Escherichia coli muk mutants by mutations in topoisomerase I. Proc. Natl. Acad. Sci. USA 97: 1671-1676 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»