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J. Bacteriol., 07 1996, 3967-3970, Vol 178, No. 13
Copyright © 1996, American Society for Microbiology

Use of PCR to isolate genes encoding sigma54-dependent activators from diverse bacteria

RI Kaufman and BT Nixon
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA.

Degenerate PCR probes were used to amplify gene fragments encoding the catalytic domain of sigma54-dependent transcription activators. The procedure should be widely applicable, as it recovered both known and novel gene fragments: 5 from Rhizobium meliloti, 13 from Myxococcus xanthus, and 3 from Bacillus subtilis. No fragments were obtained from Synechococcus sp. strain PCC 7002 or Saccharomyces cerevisiae.


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