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J. Bacteriol., 07 1996, 3967-3970, Vol 178, No. 13
RI Kaufman and BT Nixon
Degenerate PCR probes were used to amplify gene fragments encoding the
catalytic domain of sigma54-dependent transcription activators. The
procedure should be widely applicable, as it recovered both known and novel
gene fragments: 5 from Rhizobium meliloti, 13 from Myxococcus xanthus, and
3 from Bacillus subtilis. No fragments were obtained from Synechococcus sp.
strain PCC 7002 or Saccharomyces cerevisiae.
Copyright © 1996, American Society for Microbiology
Use of PCR to isolate genes encoding sigma54-dependent activators from diverse bacteria
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA.
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