J. Bacteriol., Jul 1996, 4039-4046, Vol 178, No. 14
Copyright © 1996, American Society for Microbiology

Engineering hybrid pseudomonads capable of utilizing a wide range of aromatic hydrocarbons and of efficient degradation of trichloroethylene

A Suyama, R Iwakiri, N Kimura, A Nishi, K Nakamura and K Furukawa
Department of Agricultural Chemistry, Kyushu University, Japan.

We constructed hybrid Pseudomonas strains in which the bphA1 gene (coding for a large subunit of biphenyl dioxygenase) is replaced with the todC1 gene (coding for a large subunit of toluene dioxygenase of Pseudomonas putida Fl) within chromosomal biphenyl-catabolic bph gene clusters. Such hybrid strains gained the novel capability to grow on a wide range of aromatic hydrocarbons, and, more interestingly, they degraded chloroethenes such as trichloroethylene and cis-1,2- dichloroethylene very efficiently.

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