J. Bacteriol., Jul 1996, 4084-4088, Vol 178, No. 14
Copyright © 1996, American Society for Microbiology

Tyrosine phosphorylation in Myxococcus xanthus, a multicellular prokaryote

SC Frasch and M Dworkin
Department of Microbiology, University of Minnesota, Minneapolis, 55455, USA.

Tyrosine phosphorylation is an extremely rare event in prokaryotes, occurring almost exclusively in multicellular eukaryotes. We have identified, for the first time, by the use of antiphosphotyrosine monoclonal antibody and Western blot (immunoblot) analysis, two tyrosine-phosphorylated membrane proteins in the multicellular prokaryote Myxococcus xanthus. The pattern of tyrosine phosphorylation was shown to change during development, indicating a possible role for this regulatory modification during two stages of development, i.e., aggregation and sporulation. Furthermore, the altered pattern of tyrosine phosphorylation observed in a variety of signaling mutants was shown to differ from that observed in the wild type, suggesting further the possible involvement of tyrosine phosphorylation during the development program.

This article has been cited by other articles:

• Mijakovic, I., Petranovic, D., Macek, B., Cepo, T., Mann, M., Davies, J., Jensen, P. R., Vujaklija, D. (2006). Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. Nucleic Acids Res 34: 1588-1596 [Abstract] [Full Text]  
• Moraleda-Munoz, A., Carrero-Lerida, J., Perez, J., Munoz-Dorado, J. (2003). Role of Two Novel Two-Component Regulatory Systems in Development and Phosphatase Expression in Myxococcus xanthus. J. Bacteriol. 185: 1376-1383 [Abstract] [Full Text]  
• Kimura, Y., Nakano, H., Terasaka, H., Takegawa, K. (2001). Myxococcus xanthus mokA Encodes a Histidine Kinase-Response Regulator Hybrid Sensor Required for Development and Osmotic Tolerance. J. Bacteriol. 183: 1140-1146 [Abstract] [Full Text]  
• Jain, R., Inouye, S. (1998). Inhibition of Development of Myxococcus xanthus by Eukaryotic Protein Kinase Inhibitors. J. Bacteriol. 180: 6544-6550 [Abstract] [Full Text]  
• Pope, M. K., Green, B., Westpheling, J. (1998). The bldB Gene Encodes a Small Protein Required for Morphogenesis, Antibiotic Production, and Catabolite Control in Streptomyces coelicolor. J. Bacteriol. 180: 1556-1562 [Abstract] [Full Text]