J. Bacteriol., Sep 1996, 5112-5120, Vol 178, No. 17
Copyright © 1996, American Society for Microbiology

Identification of sigma S-regulated genes in Salmonella typhimurium: complementary regulatory interactions between sigma S and cyclic AMP receptor protein

FC Fang, CY Chen, DG Guiney and Y Xu
Department of Medicine, University of Colorado Health Sciences Center, Denver 80262, USA.

sigma S (RpoS)-regulated lacZ transcriptional fusions in Salmonella typhimurium were identified from a MudJ transposon library by placing the rpoS gene under the control of the araBAD promoter and detecting lacZ expression in the presence or absence of arabinose supplementation. Western blot (immunoblot) analysis of bacteria carrying PBAD::rpoS demonstrated arabinose-dependent rpoS expression during all phases of growth. sigma S-dependent gene expression of individual gene fusions was confirmed by P22-mediated transduction of the MudJ insertions into wild-type or rpoS backgrounds. Analysis of six insertions revealed the known sigma S-regulated gene otsA, as well as five novel loci. Each of these genes is maximally expressed in stationary phase, and all but one show evidence of cyclic AMP receptor protein-dependent repression during logarithmic growth which is relieved in stationary phase. For these genes, as well as for the sigma S-regulated spvB plasmid virulence gene, a combination of rpoS overexpression and crp inactivation can result in high-level expression during logarithmic growth. The approach used to identify sigma S- regulated genes in this study provides a general method for the identification of genes controlled by trans-acting regulatory factors.

This article has been cited by other articles:

• Rydlo, T., Rotem, S., Mor, A. (2006). Antibacterial Properties of Dermaseptin S4 Derivatives under Extreme Incubation Conditions. Antimicrob. Agents Chemother. 50: 490-497 [Abstract] [Full Text]  
• Hengge-Aronis, R. (2002). Signal Transduction and Regulatory Mechanisms Involved in Control of the {sigma}S (RpoS) Subunit of RNA Polymerase. Microbiol. Mol. Biol. Rev. 66: 373-395 [Abstract] [Full Text]  
• Cánovas, D., Fletcher, S. A., Hayashi, M., Csonka, L. N. (2001). Role of Trehalose in Growth at High Temperature of Salmonella enterica Serovar Typhimurium. J. Bacteriol. 183: 3365-3371 [Abstract] [Full Text]  
• Jorgensen, F., Leach, S., Wilde, S. J., Davies, A., Stewart, G. S. A. B., Humphrey, T. (2000). Invasiveness in chickens, stress resistance and RpoS status of wild-type Salmonella enterica subsp. enterica serovar Typhimurium definitive type 104 and serovar Enteritidis phage type 4 strains. Microbiology 146: 3227-3235 [Abstract] [Full Text]  
• Ibanez-Ruiz, M., Robbe-Saule, V., Hermant, D., Labrude, S., Norel, F. (2000). Identification of RpoS (sigma S)-Regulated Genes in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 182: 5749-5756 [Abstract] [Full Text]  
• Rava, P. S., Somma, L., Steinman, H. M. (1999). Identification of a Regulator That Controls Stationary-Phase Expression of Catalase-Peroxidase in Caulobacter crescentus. J. Bacteriol. 181: 6152-6159 [Abstract] [Full Text]  
• Cunning, C., Elliott, T. (1999). RpoS Synthesis Is Growth Rate Regulated in Salmonella typhimurium, but Its Turnover Is Not Dependent on Acetyl Phosphate Synthesis or PTS Function. J. Bacteriol. 181: 4853-4862 [Abstract] [Full Text]  
• Schellhorn, H. E., Audia, J. P., Wei, L. I. C., Chang, L. (1998). Identification of Conserved, RpoS-Dependent Stationary-Phase Genes of Escherichia coli. J. Bacteriol. 180: 6283-6291 [Abstract] [Full Text]  
• Mukherjee, A., Cui, Y., Ma, W., Liu, Y., Ishihama, A., Eisenstark, A., Chatterjee, A. K. (1998). RpoS (Sigma-S) Controls Expression of rsmA, a Global Regulator of Secondary Metabolites, Harpin, and Extracellular Proteins in Erwinia carotovora. J. Bacteriol. 180: 3629-3634 [Abstract] [Full Text]  
• Ahmer, B. M. M., van Reeuwijk, J., Timmers, C. D., Valentine, P. J., Heffron, F. (1998). Salmonella typhimurium Encodes an SdiA Homolog, a Putative Quorum Sensor of the LuxR Family, That Regulates Genes on the Virulence Plasmid. J. Bacteriol. 180: 1185-1193 [Abstract] [Full Text]