J. Bacteriol., 09 1996, 5533-5536, Vol 178, No. 18
Copyright © 1996, American Society for Microbiology

Positions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site

CE Pena, JE Stoner and GF Hatfull
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA.

Mycobacteriophage L5 integrates into the genome of Mycobacterium smegmatis via site-specific recombination between the phage attP site and the bacterial attB site. These two sites have a 43-bp common core sequence within which strand exchange occurs and which overlaps a tRNAGly gene at attB. We show here that a 29-bp segment of DNA is necessary and sufficient for attB function and identify the positions of strand exchange.

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