J. Bacteriol., 09 1996, 5546-5549, Vol 178, No. 18
Copyright © 1996, American Society for Microbiology

Physical and genetic chromosomal map of an M type 1 strain of Streptococcus pyogenes

AN Suvorov and JJ Ferretti
Institute of Experimental Medicine, Pavlova, St. Petersburg, Russia.

A physical map of the chromosome of an M type 1 strain of Streptococcus pyogenes was constructed following digestion with three different restriction enzymes, SmaI, SfiI, and SgrAI, and separation and analysis of fragments by pulsed-field gel electrophoresis. The genome size of this strain was estimated to be 1,920 kb. By employing Southern hybridization and PCR analysis, 36 genes were located on the map.

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