J. Bacteriol., Oct 1996, 5644-5651, Vol 178, No. 19
Copyright © 1996, American Society for Microbiology

New compatible solutes related to Di-myo-inositol-phosphate in members of the order Thermotogales

LO Martins, LS Carreto, MS Da Costa and H Santos
Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Portugal.

The accumulation of intracellular organic solutes was examined in six species of the order Thermotogales by nuclear magnetic resonance spectroscopy. The newly discovered compounds di-2-O-beta-mannosyl-di- myo-inositol-1,1'(3,3')-phosphate and di-myo-inositol-1,3'-phosphate were identified in Thermotoga maritima and Thermotoga neapolitana. In the latter species, at the optimum temperature and salinity the organic solute pool was composed of di-myo-inositol-1,1'(3,3')-phosphate, beta- glutamate, and alpha-glutamate in addition to di-myo-inositol-1,3'- phosphate and di-2-O-beta-mannosyl-di-myo-inositol-1,1'(3,3')- phosphate. The concentrations of the last two solutes increased dramatically at supraoptimal growth temperatures, whereas beta- glutamate increased mainly in response to a salinity stress. Nevertheless, di-myo-inositol-1,1'(3,3')-phosphate was the major compatible solute at salinities above the optimum for growth. The amino acids alpha-glutamate and proline were identified under optimum growth conditions in Thermosipho africanus, and beta-mannosylglycerate, trehalose, and glycine betaine were detected in Petrotoga miotherma. Organic solutes were not detected, under optimum growth conditions, in Thermotoga thermarum and Fervidobacterium islandicum, which have a low salt requirement or none.

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