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J. Bacteriol., 10 1996, 5787-5789, Vol 178, No. 19
Copyright © 1996, American Society for Microbiology

Effect of traY amber mutations on F-plasmid traY promoter activity in vivo

PM Silverman and A Sholl
Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA. SILVERMANP@OMRF.UOKHSC.EDU

We have examined the effect of the F plasmid TraY protein on tra gene expression in vivo. Expression was assayed as alkaline phosphatase activity in cells containing a traY phi(traA'-'phoA)hyb operon under traY promoter control. Amber mutations in traY significantly reduced alkaline phosphatase activity. Since nonsense polarity effects were minimal, if they occurred at all, these data provide the first direct evidence that TraY regulates tra gene expression.


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Copyright © 1996 by the American Society for Microbiology. All rights reserved.