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J. Bacteriol., Oct 1996, 5797-5802, Vol 178, No. 19
Copyright © 1996, American Society for Microbiology

Molecular, genetic, and biochemical characterization of the serC gene of Methanosarcina barkeri Fusaro

WW Metcalf, JK Zhang, X Shi and RS Wolfe
Department of Microbiology, University of Illinois, Urbana 61801, USA. bill_metcalf@qms1.life.uiuc.edu

The Methanosarcina barkeri serC gene, encoding phosphoserine aminotransferase, was cloned by complementation of an Escherichia coli serC mutant, and its nucleotide sequence was determined. The M. barkeri SerC protein shares significant homology with other known SerC proteins. E. coli serC hosts carrying the cloned gene express phosphoserine aminotransferase activity, verifying the function of this gene.


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