J. Bacteriol., Oct 1996, 5822-5825, Vol 178, No. 19
Copyright © 1996, American Society for Microbiology

Positive regulation by nitrite of the nitrate assimilation operon in the cyanobacteria Synechococcus sp. strain PCC 7942 and Plectonema boryanum

H Kikuchi, M Aichi, I Suzuki and T Omato
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Japan.

In the absence of fixation of ammonium to glutamine, nitrate and nitrite activated transcription of the nitrate assimilation (nirA- nrtABCD-narB) operon of Synechococcus sp. strain PCC 7942. In a nitrate reductase-deficient mutant, only nitrite activated transcription, indicating that nitrite is the actual activator of the operon. Nitrate and nitrite were also found to activate the transcription of a nitrate assimilation operon in the filamentous nonheterocystous nitrogen-fixing cyanobacterium Plectonema boryanum.

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