J. Bacteriol., 01 1996, 441-446, Vol 178, No. 2
Copyright © 1996, American Society for Microbiology

Cell wall sorting of lipoproteins in Staphylococcus aureus

WW Navarre, S Daefler and O Schneewind
Department of Microbiology and Immunology, UCLA School of Medicine 90024, USA.

Many surface proteins are thought to be anchored to the cell wall of gram-positive organisms via their C termini, while the N-terminal domains of these molecules are displayed on the bacterial surface. Cell wall anchoring of surface proteins in Staphylococcus aureus requires both an N-terminal leader peptide and a C-terminal cell wall sorting signal. By fusing the cell wall sorting of protein A to the C terminus of staphylococcal beta-lactamase, we demonstrate here that lipoproteins can also be anchored to the cell wall of S. aureus. The topology of cell wall-anchored beta-lactamase is reminiscent of that described for Braun's murein lipoprotein in that the N terminus of the polypeptide chain is membrane anchored whereas the C-terminal end is tethered to the bacterial cell wall.

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