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J. Bacteriol., 01 1996, 560-563, Vol 178, No. 2
Copyright © 1996, American Society for Microbiology

A Bacillus subtilis malate dehydrogenase gene

S Jin, M De Jesus-Berrios and AL Sonenshein
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.

A Bacillus subtilis gene for malate dehydrogenase (citH) was found downstream of genes for citrate synthase and isocitrate dehydrogenase. Disruption of citH caused partial auxotrophy for aspartate and a requirement for aspartate during sporulation. In the absence of aspartate, citH mutant cells were blocked at a late stage of spore formation.


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