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J. Bacteriol., Jan 1996, 564-570, Vol 178, No. 2
Copyright © 1996, American Society for Microbiology

The lipooligosaccharides of Haemophilus ducreyi are highly sialylated

W Melaugh, AA Campagnari and BW Gibson
Department of Pharmaceutical Chemistry, University of California, San Francisco 94143-0446, USA.

The major lipooligosaccharides of the sexually transmitted pathogen Haemophilus ducreyi 35000 have been previously found to terminate in N- acetyllactosamine and sialyl-N-acetyllactosamine, Neu5Ac alpha 2-->3Gal beta 1-->4GlcNAc (W. Melaugh, N. J. Phillips, A. A. Campagnari, M. V. Tullius, and B. W. Gibson, Biochemistry 33: 13070-13078, 1994). In this study, mass spectrometry and composition analyses have shown that the lipooligosaccharides from three other H. ducreyi strains also contain N- acetyllactosamine and are highly sialylated (approximately 30 to 50%), although one African strain was found to contain neither of these structural features.


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