J. Bacteriol., 01 1996, 571-573, Vol 178, No. 2
Copyright © 1996, American Society for Microbiology

Isolation of Vibrio harveyi acyl carrier protein and the fabG, acpP, and fabF genes involved in fatty acid biosynthesis

Z Shen and DM Byers
Department of Pediatrics, Atlantic Resarch Centre, Dalhousie University, Halifax, Nova Scotia, Canada.

We report the isolation of Vibrio harveyi acyl carrier protein (ACP) and cloning of a 3,973-bp region containing the fabG (encoding 3- ketoacyl-ACP reductase, 25.5 kDa), acpP (encoding ACP, 8.7 kDa), fabF (encoding 3-ketoacyl-ACP synthase II, 43.1 kDa), and pabC (encoding aminodeoxychorismate lyase, 29.9 kDa) genes. Predicted amino acid sequences were, respectively, 78, 86, 76, and 35% identical to those of the corresponding Escherichia coli proteins. Five of the 11 sequence differences between V. harveyi and E. coli ACP were nonconservative amino acid differences concentrated in a loop region between helices I and II.

This article has been cited by other articles:

• McAllister, K. A., Peery, R. B., Zhao, G. (2006). Acyl Carrier Protein Synthases from Gram-Negative, Gram-Positive, and Atypical Bacterial Species: Biochemical and Structural Properties and Physiological Implications. J. Bacteriol. 188: 4737-4748 [Abstract] [Full Text]  
• Steffensky, M., Mühlenweg, A., Wang, Z.-X., Li, S.-M., Heide, L. (2000). Identification of the Novobiocin Biosynthetic Gene Cluster of Streptomyces spheroides NCIB 11891. Antimicrob. Agents Chemother. 44: 1214-1222 [Abstract] [Full Text]  
• Allen, E. E., Bartlett, D. H. (2000). FabF Is Required for Piezoregulation of cis-Vaccenic Acid Levels and Piezophilic Growth of the Deep-Sea Bacterium Photobacterium profundum Strain SS9. J. Bacteriol. 182: 1264-1271 [Abstract] [Full Text]  
• Kutchma, A. J., Hoang, T. T., Schweizer, H. P. (1999). Characterization of a Pseudomonas aeruginosa Fatty Acid Biosynthetic Gene Cluster: Purification of Acyl Carrier Protein (ACP) and Malonyl-Coenzyme A:ACP Transacylase (FabD). J. Bacteriol. 181: 5498-5504 [Abstract] [Full Text]  
• Zhang, Y., Cronan, J. E. Jr. (1998). Transcriptional Analysis of Essential Genes of the Escherichia coli Fatty Acid Biosynthesis Gene Cluster by Functional Replacement with the Analogous Salmonella typhimurium Gene Cluster. J. Bacteriol. 180: 3295-3303 [Abstract] [Full Text]  
• McAllister, K. A., Peery, R. B., Meier, T. I., Fischl, A. S., Zhao, G. (2000). Biochemical and Molecular Analyses of the Streptococcus pneumoniae Acyl Carrier Protein Synthase, an Enzyme Essential for Fatty Acid Biosynthesis. J. Biol. Chem. 275: 30864-30872 [Abstract] [Full Text]  
• Flaman, A. S., Chen, J. M., Van Iderstine, S. C., Byers, D. M. (2001). Site-directed Mutagenesis of Acyl Carrier Protein (ACP) Reveals Amino Acid Residues Involved in ACP Structure and Acyl-ACP Synthetase Activity. J. Biol. Chem. 276: 35934-35939 [Abstract] [Full Text]