This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dunn, M. F.
Right arrow Articles by Mora, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dunn, M. F.
Right arrow Articles by Mora, J.

 Previous Article  |  Next Article 

J. Bacteriol., Oct 1996, 5960-5970, Vol 178, No. 20
Copyright © 1996, American Society for Microbiology

Pyruvate carboxylase from Rhizobium etli: mutant characterization, nucleotide sequence, and physiological role

MF Dunn, S Encarnacion, G Araiza, MC Vargas, A Davalos, H Peralta, Y Mora and J Mora
Departamento de Ecologia Molecular, Centro de Investigacion sobre Fijacion de Nitrogeno, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos. mike@cifn.unam.mx

Pyruvate carboxylase (PYC), a biotin-dependent enzyme which catalyzes the conversion of pyruvate to oxaloacetate, was hypothesized to play an important anaplerotic role in the growth of Rhizobium etli during serial subcultivation in minimal media containing succinate (S. Encarnacion, M. Dunn, K. Willms, and J. Mora, J. Bacteriol. 177:3058- 3066, 1995). R. etli and R. tropici pyc::Tn5-mob mutants were selected for their inability to grow in minimal medium with pyruvate as a sole carbon source. During serial subcultivation in minimal medium containing 30 mM succinate, the R. etli parent and pyc mutant strains exhibited similar decreases in growth rate with each subculture. Supplementation of the medium with biotin prevented the growth decrease of the parent but not the mutant strain, indicating that PYC was necessary for the growth of R. etli under these conditions. The R. tropici pyc mutant grew normally in subcultures regardless of biotin supplementation. The symbiotic phenotypes of the pyc mutants from both species were similar to those of the parent strains. The R. etli pyc was cloned, sequenced, and found to encode a 126-kDa protein of 1,154 amino acids. The deduced amino acid sequence is highly homologous to other PYC sequences, and the catalytic domains involved in carboxylation, pyruvate binding, and biotinylation are conserved. The sequence and biochemical data show that the R. etli PYC is a member of the alpha4, homotetrameric, acetyl coenzyme A-activated class of PYCs.


This article has been cited by other articles:

  • Flores, C.-L., Gancedo, C. (2005). Yarrowia lipolytica Mutants Devoid of Pyruvate Carboxylase Activity Show an Unusual Growth Phenotype. Eukaryot Cell 4: 356-364 [Abstract] [Full Text]  
  • Dunn, M. F., Araiza, G., Mora, J. (2004). Biochemical characterization of a Rhizobium etli monovalent cation-stimulated acyl-coenzyme A carboxylase with a high substrate specificity constant for propionyl-coenzyme A. Microbiology 150: 399-406 [Abstract] [Full Text]  
  • del Carmen Vargas, M., Encarnacion, S., Davalos, A., Reyes-Perez, A., Mora, Y., Garcia-de los Santos, A., Brom, S., Mora, J. (2003). Only one catalase, katG, is detectable in Rhizobium etli, and is encoded along with the regulator OxyR on a plasmid replicon. Microbiology 149: 1165-1176 [Abstract] [Full Text]  
  • March, J. C., Eiteman, M. A., Altman, E. (2002). Expression of an Anaplerotic Enzyme, Pyruvate Carboxylase, Improves Recombinant Protein Production in Escherichia coli. Appl. Environ. Microbiol. 68: 5620-5624 [Abstract] [Full Text]  
  • Encarnacion, S., del Carmen Vargas, M., Dunn, M. F., Davalos, A., Mendoza, G., Mora, Y., Mora, J. (2002). AniA Regulates Reserve Polymer Accumulation and Global Protein Expression in Rhizobium etli. J. Bacteriol. 184: 2287-2295 [Abstract] [Full Text]  
  • Dunn, M. F., Araiza, G., Encarnacion, S., del Carmen Vargas, M., Mora, J. (2002). Effect of aniA (Carbon Flux Regulator) and phaC (Poly-{beta}-Hydroxybutyrate Synthase) Mutations on Pyruvate Metabolism in Rhizobium etli. J. Bacteriol. 184: 2296-2299 [Abstract] [Full Text]  
  • Gokarn, R. R., Eiteman, M. A., Altman, E. (2000). Metabolic Analysis of Escherichia coli in the Presence and Absence of the Carboxylating Enzymes Phosphoenolpyruvate Carboxylase and Pyruvate Carboxylase. Appl. Environ. Microbiol. 66: 1844-1850 [Abstract] [Full Text]  
  • Wang, H., O'Sullivan, D. J., Baldwin, K. A., McKay, L. L. (2000). Cloning, Sequencing, and Expression of the Pyruvate Carboxylase Gene in Lactococcus lactis subsp. lactis C2. Appl. Environ. Microbiol. 66: 1223-1227 [Abstract] [Full Text]  
  • Mukhopadhyay, B., Stoddard, S. F., Wolfe, R. S. (1998). Purification, Regulation, and Molecular and Biochemical Characterization of Pyruvate Carboxylase from Methanobacterium thermoautotrophicum Strain Delta H. J. Biol. Chem. 273: 5155-5166 [Abstract] [Full Text]  
  • Volpin, H., Phillips, D. A. (1998). Respiratory Elicitors from Rhizobium meliloti Affect Intact Alfalfa Roots. Plant Physiol. 116: 777-783 [Abstract] [Full Text]  
  • Jitrapakdee, S., Booker, G. W., Cassady, A. I., Wallace, J. C. (1997). The Rat Pyruvate Carboxylase Gene Structure. ALTERNATE PROMOTERS GENERATE MULTIPLE TRANSCRIPTS WITH THE 5'-END HETEROGENEITY. J. Biol. Chem. 272: 20522-20530 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»