J. Bacteriol., Oct 1996, 6067-6069, Vol 178, No. 20
Copyright © 1996, American Society for Microbiology

Secondary structure of the outer membrane proteins OmpA of Escherichia coli and OprF of Pseudomonas aeruginosa

E Sugawara, M Steiert, S Rouhani and H Nikaido
Department of Molecular and Cell Biology, University of California, Berkeley, USA.

When purified without the use of ionic detergents, both OmpA and OprF proteins contained nearly 20% alpha-helical structures, which disappeared completely upon the addition of sodium dodecyl sulfate. This result suggests that the proteins fold in a similar manner, with an N-terminal, membrane-spanning beta-barrel domain and a C-terminal, globular, periplasmic domain.

This article has been cited by other articles:

• Kaserer, W. A., Jiang, X., Xiao, Q., Scott, D. C., Bauler, M., Copeland, D., Newton, S. M. C., Klebba, P. E. (2008). Insight from TonB Hybrid Proteins into the Mechanism of Iron Transport through the Outer Membrane. J. Bacteriol. 190: 4001-4016 [Abstract] [Full Text]  
• Kumagai, Y., Huang, H., Rikihisa, Y. (2008). Expression and Porin Activity of P28 and OMP-1F during Intracellular Ehrlichia chaffeensis Development. J. Bacteriol. 190: 3597-3605 [Abstract] [Full Text]  
• Huang, H., Wang, X., Kikuchi, T., Kumagai, Y., Rikihisa, Y. (2007). Porin Activity of Anaplasma phagocytophilum Outer Membrane Fraction and Purified P44. J. Bacteriol. 189: 1998-2006 [Abstract] [Full Text]  
• Zakharian, E., Reusch, R. N. (2007). Haemophilus influenzae Outer Membrane Protein P5 Is Associated with Inorganic Polyphosphate and Polyhydroxybutyrate. Biophys. J 92: 588-593 [Abstract] [Full Text]  
• Zakharian, E., Reusch, R. N. (2006). Pore Characteristics of Nontypeable Haemophilus influenzae Outer Membrane Protein P5 in Planar Lipid Bilayers. Biophys. J 91: 3242-3248 [Abstract] [Full Text]  
• Sugawara, E., Nestorovich, E. M., Bezrukov, S. M., Nikaido, H. (2006). Pseudomonas aeruginosa Porin OprF Exists in Two Different Conformations. J. Biol. Chem. 281: 16220-16229 [Abstract] [Full Text]  
• Ng, T. W., Akman, L., Osisami, M., Thanassi, D. G. (2004). The Usher N Terminus Is the Initial Targeting Site for Chaperone-Subunit Complexes and Participates in Subsequent Pilus Biogenesis Events. J. Bacteriol. 186: 5321-5331 [Abstract] [Full Text]  
• Nikaido, H. (2003). Molecular Basis of Bacterial Outer Membrane Permeability Revisited. Microbiol. Mol. Biol. Rev. 67: 593-656 [Abstract] [Full Text]  
• Singh, S. P., Williams, Y. U., Miller, S., Nikaido, H. (2003). The C-Terminal Domain of Salmonella enterica Serovar Typhimurium OmpA Is an Immunodominant Antigen in Mice but Appears To Be Only Partially Exposed on the Bacterial Cell Surface. Infect. Immun. 71: 3937-3946 [Abstract] [Full Text]  
• Thanassi, D. G., Stathopoulos, C., Dodson, K., Geiger, D., Hultgren, S. J. (2002). Bacterial Outer Membrane Ushers Contain Distinct Targeting and Assembly Domains for Pilus Biogenesis. J. Bacteriol. 184: 6260-6269 [Abstract] [Full Text]  
• Sze, C. C., Laurie, A. D., Shingler, V. (2001). In Vivo and In Vitro Effects of Integration Host Factor at the DmpR-Regulated {sigma}54-Dependent Po Promoter. J. Bacteriol. 183: 2842-2851 [Abstract] [Full Text]  
• Sarand, I., Skärfstad, E., Forsman, M., Romantschuk, M., Shingler, V. (2001). Role of the DmpR-Mediated Regulatory Circuit in Bacterial Biodegradation Properties in Methylphenol-Amended Soils. Appl. Environ. Microbiol. 67: 162-171 [Abstract] [Full Text]  
• Carmona, M., Rodríguez, M. J., Martínez-Costa, O., de Lorenzo, V. (2000). In Vivo and In Vitro Effects of (p)ppGpp on the sigma 54 Promoter Pu of the TOL Plasmid of Pseudomonas putida. J. Bacteriol. 182: 4711-4718 [Abstract] [Full Text]  
• Skärfstad, E., O'Neill, E., Garmendia, J., Shingler, V. (2000). Identification of an Effector Specificity Subregion within the Aromatic-Responsive Regulators DmpR and XylR by DNA Shuffling. J. Bacteriol. 182: 3008-3016 [Abstract] [Full Text]  
• Novotny, L. A., Jurcisek, J. A., Pichichero, M. E., Bakaletz, L. O. (2000). Epitope Mapping of the Outer Membrane Protein P5-Homologous Fimbrin Adhesin of Nontypeable Haemophilus influenzae. Infect. Immun. 68: 2119-2128 [Abstract] [Full Text]  
• Arora, A., Rinehart, D., Szabo, G., Tamm, L. K. (2000). Refolded Outer Membrane Protein A of Escherichia coli Forms Ion Channels with Two Conductance States in Planar Lipid Bilayers. J. Biol. Chem. 275: 1594-1600 [Abstract] [Full Text]  
• Webb, D. C., Cripps, A. W. (2000). A P5 Peptide That Is Homologous to Peptide 10 of OprF from Pseudomonas aeruginosa Enhances Clearance of Nontypeable Haemophilus influenzae from Acutely Infected Rat Lung in the Absence of Detectable Peptide-Specific Antibody. Infect. Immun. 68: 377-381 [Abstract] [Full Text]  
• Carmona, M., de Lorenzo, V., Bertoni, G. (1999). Recruitment of RNA Polymerase Is a Rate-limiting Step for the Activation of the sigma 54 Promoter Pu of Pseudomonas putida. J. Biol. Chem. 274: 33790-33794 [Abstract] [Full Text]  
• Maurer, J., Jose, J., Meyer, T. F. (1999). Characterization of the Essential Transport Function of the AIDA-I Autotransporter and Evidence Supporting Structural Predictions. J. Bacteriol. 181: 7014-7020 [Abstract] [Full Text]  
• Murphy, T. F., Kirkham, C., DeNardin, E., Sethi, S. (1999). Analysis of Antigenic Structure and Human Immune Response to Outer Membrane Protein CD of Moraxella catarrhalis. Infect. Immun. 67: 4578-4585 [Abstract] [Full Text]  
• Koebnik, R. (1999). Structural and Functional Roles of the Surface-Exposed Loops of the beta -Barrel Membrane Protein OmpA from Escherichia coli. J. Bacteriol. 181: 3688-3694 [Abstract] [Full Text]  
• Rosenqvist, E., Musacchio, A., Aase, A., Hoiby, E. A., Namork, E., Kolberg, J., Wedege, E., Delvig, A., Dalseg, R., Michaelsen, T. E., Tommassen, J. (1999). Functional Activities and Epitope Specificity of Human and Murine Antibodies against the Class 4 Outer Membrane Protein (Rmp) of Neisseria meningitidis. Infect. Immun. 67: 1267-1276 [Abstract] [Full Text]  
• Fajardo, D. A., Cheung, J., Ito, C., Sugawara, E., Nikaido, H., Misra, R. (1998). Biochemistry and Regulation of a Novel Escherichia coli K-12 Porin Protein, OmpG, Which Produces Unusually Large Channels. J. Bacteriol. 180: 4452-4459 [Abstract] [Full Text]  
• Senaratne, R. H., Mobasheri, H., Papavinasasundaram, K. G., Jenner, P., Lea, E. J. A., Draper, P. (1998). Expression of a Gene for a Porin-Like Protein of the OmpA Family from Mycobacterium tuberculosis H37Rv. J. Bacteriol. 180: 3541-3547 [Abstract] [Full Text]  
• Thanassi, D. G., Saulino, E. T., Lombardo, M.-J., Roth, R., Heuser, J., Hultgren, S. J. (1998). The PapC usher forms an oligomeric channel: Implications for pilus biogenesis across the outer membrane. Proc. Natl. Acad. Sci. USA 95: 3146-3151 [Abstract] [Full Text]