J. Bacteriol., 11 1996, 6357-6360, Vol 178, No. 21
Copyright © 1996, American Society for Microbiology

Apramycin resistance as a selective marker for gene transfer in mycobacteria

E Paget and J Davies
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.

We have explored the potential of using the apramycin resistance gene as a marker in mycobacterial gene transfer studies. Shuttle plasmids available for both electroporation and conjugation studies have been constructed, and we have successfully validated the use of the apramycin resistance gene as a component of cloning vectors for Mycobacterium smegmatis, M. bovis BCG, and M. tuberculosis.

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