J. Bacteriol., Nov 1996, 6369-6373, Vol 178, No. 21
Copyright © 1996, American Society for Microbiology

Transcriptional regulation of Salmonella virulence: a PhoQ periplasmic domain mutation results in increased net phosphotransfer to PhoP

JS Gunn, EL Hohmann and SI Miller
Department of Medicine, University of Washington, Seattle 98195, USA.

A mutation in the phoP/phoQ locus (pho-24) that results in unregulated expression of PhoP-activated genes (phenotype PhoP constitutive [PhoP(c)]) was mapped to phoQ. Change of a Thr to Ile at position 48 of PhoQ was responsible for the PhoP(c) phenotype (attenuation of mouse virulence, defects in epithelial cell invasion, and macrophage spacious phagosome formation). PhoP phosphorylation by membrane extracts required PhoQ, and PhoQ Ile-48-containing membranes demonstrated increased net phosphorylation of PhoP.

This article has been cited by other articles:

• Bergman, M. A., Cummings, L. A., Barrett, S. L. R., Smith, K. D., Lara, J. C., Aderem, A., Cookson, B. T. (2005). CD4+ T Cells and Toll-Like Receptors Recognize Salmonella Antigens Expressed in Bacterial Surface Organelles. Infect. Immun. 73: 1350-1356 [Abstract] [Full Text]  
• Prouty, A. M., Brodsky, I. E., Falkow, S., Gunn, J. S. (2004). Bile-salt-mediated induction of antimicrobial and bile resistance in Salmonella typhimurium. Microbiology 150: 775-783 [Abstract] [Full Text]  
• Baud, D., Benyacoub, J., Revaz, V., Kok, M., Ponci, F., Bobst, M., Curtiss, R. III, De Grandi, P., Nardelli-Haefliger, D. (2004). Immunogenicity against Human Papillomavirus Type 16 Virus-Like Particles Is Strongly Enhanced by the PhoPc Phenotype in Salmonella enterica Serovar Typhimurium. Infect. Immun. 72: 750-756 [Abstract] [Full Text]  
• Appleman, J. A., Chen, L.-L., Stewart, V. (2003). Probing Conservation of HAMP Linker Structure and Signal Transduction Mechanism through Analysis of Hybrid Sensor Kinases. J. Bacteriol. 185: 4872-4882 [Abstract] [Full Text]  
• Sanowar, S., Martel, A., Moual, H. L. (2003). Mutational Analysis of the Residue at Position 48 in the Salmonella enterica Serovar Typhimurium PhoQ Sensor Kinase. J. Bacteriol. 185: 1935-1941 [Abstract] [Full Text]  
• Regelmann, A. G., Lesley, J. A., Mott, C., Stokes, L., Waldburger, C. D. (2002). Mutational Analysis of the Escherichia coli PhoQ Sensor Kinase: Differences with the Salmonella enterica Serovar Typhimurium PhoQ Protein and in the Mechanism of Mg2+ and Ca2+ Sensing. J. Bacteriol. 184: 5468-5478 [Abstract] [Full Text]  
• Takaya, A., Tomoyasu, T., Tokumitsu, A., Morioka, M., Yamamoto, T. (2002). The ATP-Dependent Lon Protease of Salmonella enterica Serovar Typhimurium Regulates Invasion and Expression of Genes Carried on Salmonella Pathogenicity Island 1. J. Bacteriol. 184: 224-232 [Abstract] [Full Text]  
• Fahlen, T. F., Wilson, R. L., Boddicker, J. D., Jones, B. D. (2001). Hha Is a Negative Modulator of Transcription of hilA, the Salmonella enterica Serovar Typhimurium Invasion Gene Transcriptional Activator. J. Bacteriol. 183: 6620-6629 [Abstract] [Full Text]  
• Lucas, R. L., Lee, C. A. (2001). Roles of hilC and hilD in Regulation of hilA Expression in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 183: 2733-2745 [Abstract] [Full Text]  
• Groisman, E. A. (2001). The Pleiotropic Two-Component Regulatory System PhoP-PhoQ. J. Bacteriol. 183: 1835-1842 [Full Text]  
• Montagne, M., Martel, A., Le Moual, H. (2001). Characterization of the Catalytic Activities of the PhoQ Histidine Protein Kinase of Salmonella enterica Serovar Typhimurium. J. Bacteriol. 183: 1787-1791 [Abstract] [Full Text]  
• Gronow, S., Brade, H. (2001). Invited review: Lipopolysaccharide biosynthesis: which steps do bacteria need to survive?. Innate Immunity 7: 3-23 [Abstract]  
• Niedergang, F., Sirard, J.-C., Blanc, C. T., Kraehenbuhl, J.-P. (2000). Entry and survival of Salmonella typhimurium in dendritic cells and presentation of recombinant antigens do not require macrophage-specific virulence factors. Proc. Natl. Acad. Sci. USA 97: 14650-14655 [Abstract] [Full Text]  
• Prouty, A. M., Gunn, J. S. (2000). Salmonella enterica Serovar Typhimurium Invasion Is Repressed in the Presence of Bile. Infect. Immun. 68: 6763-6769 [Abstract] [Full Text]  
• Gunn, J. S., Ryan, S. S., Van Velkinburgh, J. C., Ernst, R. K., Miller, S. I. (2000). Genetic and Functional Analysis of a PmrA-PmrB-Regulated Locus Necessary for Lipopolysaccharide Modification, Antimicrobial Peptide Resistance, and Oral Virulence of Salmonella enterica Serovar Typhimurium. Infect. Immun. 68: 6139-6146 [Abstract] [Full Text]  
• Guina, T., Yi, E. C., Wang, H., Hackett, M., Miller, S. I. (2000). A PhoP-Regulated Outer Membrane Protease of Salmonella enterica Serovar Typhimurium Promotes Resistance to Alpha-Helical Antimicrobial Peptides. J. Bacteriol. 182: 4077-4086 [Abstract] [Full Text]  
• Gunn, J. S., Ernst, R. K., McCoy, A. J., Miller, S. I. (2000). Constitutive Mutations of the Salmonella enterica Serovar Typhimurium Transcriptional Virulence Regulator phoP. Infect. Immun. 68: 3758-3762 [Abstract] [Full Text]  
• Lucas, R. L., Lostroh, C. P., DiRusso, C. C., Spector, M. P., Wanner, B. L., Lee, C. A. (2000). Multiple Factors Independently Regulate hilA and Invasion Gene Expression in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 182: 1872-1882 [Abstract] [Full Text]  
• Ernst, R. K., Yi, E. C., Guo, L., Lim, K. B., Burns, J. L., Hackett, M., Miller, S. I. (1999). Specific Lipopolysaccharide Found in Cystic Fibrosis Airway Pseudomonas aeruginosa. Science 286: 1561-1565 [Abstract] [Full Text]  
• Kato, A., Tanabe, H., Utsumi, R. (1999). Molecular Characterization of the PhoP-PhoQ Two-Component System in Escherichia coli K-12: Identification of Extracellular Mg2+-Responsive Promoters. J. Bacteriol. 181: 5516-5520 [Abstract] [Full Text]  
• Heran Darwin, K., Miller, V. L. (1999). InvF Is Required for Expression of Genes Encoding Proteins Secreted by the SPI1 Type III Secretion Apparatus in Salmonella typhimurium. J. Bacteriol. 181: 4949-4954 [Abstract] [Full Text]  
• Benyacoub, J., Hopkins, S., Potts, A., Kelly, S., Kraehenbuhl, J.-P., Curtiss, R. III, De Grandi, P., Nardelli-Haefliger, D. (1999). The Nature of the Attenuation of Salmonella typhimurium Strains Expressing Human Papillomavirus Type 16 Virus-Like Particles Determines the Systemic and Mucosal Antibody Responses in Nasally Immunized Mice. Infect. Immun. 67: 3674-3679 [Abstract] [Full Text]  
• Rakeman, J. L., Bonifield, H. R., Miller, S. I. (1999). A HilA-Independent Pathway to Salmonella typhimurium Invasion Gene Transcription. J. Bacteriol. 181: 3096-3104 [Abstract] [Full Text]  
• van Velkinburgh, J. C., Gunn, J. S. (1999). PhoP-PhoQ-Regulated Loci Are Required for Enhanced Bile Resistance in Salmonella spp.. Infect. Immun. 67: 1614-1622 [Abstract] [Full Text]  
• Heithoff, D. M., Conner, C. P., Hentschel, U., Govantes, F., Hanna, P. C., Mahan, M. J. (1999). Coordinate Intracellular Expression of Salmonella Genes Induced during Infection. J. Bacteriol. 181: 799-807 [Abstract] [Full Text]  
• Guo, L., Lim, K. B., Gunn, J. S., Bainbridge, B., Darveau, R. P., Hackett, M., Miller, S. I. (1997). Regulation of Lipid A Modifications by Salmonella typhimurium Virulence Genes phoP-phoQ. Science 276: 250-253 [Abstract] [Full Text]