J. Bacteriol., Nov 1996, 6378-6381, Vol 178, No. 21
Copyright © 1996, American Society for Microbiology

The Tra2 core of the IncP(alpha) plasmid RP4 is required for intergeneric mating between Escherichia coli and Streptomyces lividans

LA Giebelhaus, L Frost, E Lanka, EP Gormley, JE Davies and B Leskiw
Department of Biological Sciences, University of Alberta, Edmonton, Canada.

Escherichia coli cells and Streptomyces mycelia are able to form close contacts in the absence of a conjugative system which might facilitate intergeneric plasmid transfer without the genes required for mating pair formation (Tra2) of the RP4 plasmid. The same Tra2 genes found to be essential for RP4 plasmid transfer, RSF1010 mobilization, and donor- specific phage propagation in E. coli were also required for intergeneric transfer between E. coli and Streptomyces lividans.

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