J. Bacteriol., Nov 1996, 6403-6406, Vol 178, No. 21
Copyright © 1996, American Society for Microbiology

Genetic and chemical characterization of a mutant that disrupts synthesis of the lipopolysaccharide core tetrasaccharide in Rhizobium leguminosarum

D Allaway, B Jeyaretnam, RW Carlson and PS Poole
School of Animal and Microbial Science, University of Reading, United Kingdom.

A 2-kb region that complements the Tn5-derived lipopolysaccharide (LPS) rough mutant Rhizobium leguminosarum RU301 was sequenced. Two open reading frames (ORFs) were identified. The first ORF (lpcA) is homologous to a family of bacterial sugar transferases involved in LPS core tetrasaccharide biosynthesis. ORF2 (lpcB), in which Tn5 transposed, has no significant homology to any DNA in the GenBank-EMBL databases. Chemical characterization of LPS produced by strain RU301 demonstrated that the 3-deoxy-D-manno-2-octulosonic acid (Kdo) residue which normally attaches the core tetrasaccharide to the O chain was missing, suggesting that IpcB may encode a CMP-Kdo:LPS Kdo transferase.

This article has been cited by other articles:

• Williams, A., Wilkinson, A., Krehenbrink, M., Russo, D. M., Zorreguieta, A., Downie, J. A. (2008). Glucomannan-Mediated Attachment of Rhizobium leguminosarum to Pea Root Hairs Is Required for Competitive Nodule Infection. J. Bacteriol. 190: 4706-4715 [Abstract] [Full Text]  
• Vinuesa, P., Reuhs, B. L., Breton, C., Werner, D. (1999). Identification of a Plasmid-Borne Locus in Rhizobium etli KIM5s Involved in Lipopolysaccharide O-Chain Biosynthesis and Nodulation of Phaseolus vulgaris. J. Bacteriol. 181: 5606-5614 [Abstract] [Full Text]  
• Shibayama, K., Ohsuka, S., Tanaka, T., Arakawa, Y., Ohta, M. (1998). Conserved Structural Regions Involved in the Catalytic Mechanism of Escherichia coli K-12 WaaO (RfaI). J. Bacteriol. 180: 5313-5318 [Abstract] [Full Text]  
• Kadrmas, J. L., Allaway, D., Studholme, R. E., Sullivan, J. T., Ronson, C. W., Poole, P. S., Raetz, C. R. H. (1998). Cloning and Overexpression of Glycosyltransferases That Generate the Lipopolysaccharide Core of Rhizobium leguminosarum. J. Biol. Chem. 273: 26432-26440 [Abstract] [Full Text]  
• Forsberg, L. S., Bhat, U. R., Carlson, R. W. (2000). Structural Characterization of the O-antigenic Polysaccharide of the Lipopolysaccharide from Rhizobium etli Strain CE3. A UNIQUE O-ACETYLATED GLYCAN OF DISCRETE SIZE, CONTAINING 3-O-METHYL-6-DEOXY-L-TALOSE AND 2,3,4-TRI-O-METHYL-L-FUCOSE. J. Biol. Chem. 275: 18851-18863 [Abstract] [Full Text]  
• Lerouge, I., Laeremans, T., Verreth, C., Vanderleyden, J., Van Soom, C., Tobin, A., Carlson, R. W. (2001). Identification of an ATP-binding Cassette Transporter for Export of the O-antigen across the Inner Membrane in Rhizobium etli Based on the Genetic, Functional, and Structural Analysis of an lps Mutant Deficient in O-antigen. J. Biol. Chem. 276: 17190-17198 [Abstract] [Full Text]