J. Bacteriol., 11 1996, 6644-6646, Vol 178, No. 22
Copyright © 1996, American Society for Microbiology

A novel type of pyridine nucleotide-disulfide oxidoreductase is essential for NAD+- and NADPH-dependent degradation of epoxyalkanes by Xanthobacter strain Py2

J Swaving, JA de Bont, A Westphal and A de Kok
Division of Industrial Microbiology, Department of Food Science, Wageningen Agricultural University, The Netherlands. jelto.swaving@algemeen.im.wau.nl

Epoxide degradation in cell extracts of Xanthobacter strain Py2 has been reported to be dependent on NAD+ and dithiols. This multicomponent system has now been fractionated. A key protein encoded by a DNA fragment complementing a Xanthobacter strain Py2 mutant unable to degrade epoxides was purified and analyzed. This NADP-dependent protein, a novel type of pyridine nucleotide-disulfide oxidoreductase, is essential for epoxide degradation. NADPH, acting as the physiological cofactor, replaced the dithiols in epoxide conversion.

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