J. Bacteriol., Dec 1996, 6991-6993, Vol 178, No. 23
Copyright © 1996, American Society for Microbiology

Escherichia coli rpoC397 encodes a temperature-sensitive C-terminal frameshift in the beta' subunit of RNA polymerase that blocks growth of bacteriophage P2

GE Christie, SB Cale, LA Isaksson, DJ Jin, M Xu, B Sauer and R Calendar
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298-0678, USA. christie@gems.vcu.edu.

Escherichia coli 397c is temperature sensitive for growth at 43.5 degrees C and unable to plate bacteriophage P2 at 33 degrees C. The mutation conferring these phenotypes was mapped to the rpoC gene. RNA synthesis is temperature sensitive in the mutant strain, and the beta' subunit of RNA polymerase isolated from this strain exhibits increased electrophoretic mobility. DNA sequence analysis revealed that the mutation is a deletion of 16 bp, resulting in a frameshift that leads to truncation of the beta' subunit at the carboxy terminus.

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