J. Bacteriol., 12 1996, 7014-7015, Vol 178, No. 23
Copyright © 1996, American Society for Microbiology

Significance of HPr in catabolite repression of alpha-amylase

MI Voskuil and GH Chambliss
Department of Bacteriology, University of Wisconsin-Madison, 53706, USA.

CcpA and HPr are presently the only two proteins implicated in Bacillus subtilis global carbon source catabolite repression, and the ptsH1 mutation in the gene for the HPr protein was reported to relieve catabolite repression of several genes. However, alpha-amylase synthesis by B. subtilis SA003 containing the ptsH1 mutation was repressed by glucose. Our results suggest HPr(Ser-P) may be involved in but is not required for catabolite repression of alpha-amylase, indicating that HPr(Ser-P) is not the sole signaling molecule for CcpA- mediated catabolite repression in B. subtilis.

This article has been cited by other articles:

• Deutscher, J., Francke, C., Postma, P. W. (2006). How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria. Microbiol. Mol. Biol. Rev. 70: 939-1031 [Abstract] [Full Text]  
• Küster-Schöck, E., Wagner, A., Völker, U., Hillen, W. (1999). Mutations in Catabolite Control Protein CcpA Showing Glucose-Independent Regulation in Bacillus megaterium. J. Bacteriol. 181: 7634-7638 [Abstract] [Full Text]  
• Zalieckas, J. M., Wray, L. V. Jr., Fisher, S. H. (1998). Expression of the Bacillus subtilis acsA Gene: Position and Sequence Context Affect cre-Mediated Carbon Catabolite Repression. J. Bacteriol. 180: 6649-6654 [Abstract] [Full Text]  
• Liu, X., Taber, H. W. (1998). Catabolite Regulation of the Bacillus subtilis ctaBCDEF Gene Cluster. J. Bacteriol. 180: 6154-6163 [Abstract] [Full Text]  
• Turinsky, A. J., Grundy, F. J., Kim, J.-H., Chambliss, G. H., Henkin, T. M. (1998). Transcriptional Activation of the Bacillus subtilis ackA Gene Requires Sequences Upstream of the Promoter. J. Bacteriol. 180: 5961-5967 [Abstract] [Full Text]  
• Christensen, D. P., Benson, A. K., Hutkins, R. W. (1998). Cloning and Expression of the Listeria monocytogenes Scott A ptsH and ptsI Genes, Coding for HPr and Enzyme I, Respectively, of the Phosphotransferase System. Appl. Environ. Microbiol. 64: 3147-3152 [Abstract] [Full Text]  
• Kim, J.-H., Voskuil, M. I., Chambliss, G. H. (1998). NADP, corepressor for the Bacillus catabolite control protein CcpA. Proc. Natl. Acad. Sci. USA 95: 9590-9595 [Abstract] [Full Text]  
• Jones, B. E., Dossonnet, V., Kuster, E., Hillen, W., Deutscher, J., Klevit, R. E. (1997). Binding of the Catabolite Repressor Protein CcpA to Its DNA Target Is Regulated by Phosphorylation of its Corepressor HPr. J. Biol. Chem. 272: 26530-26535 [Abstract] [Full Text]