J. Bacteriol., Dec 1996, 7304-7307, Vol 178, No. 24
Copyright © 1996, American Society for Microbiology

Inhibition of RecA-mediated cleavage in covalent dimers of UmuD

MH Lee, A Guzzo and GC Walker
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.

Disulfide-cross-linked UmuD2 derivatives were cleaved poorly upon incubation with activated RecA. Reducing the disulfide bonds prior to incubating the derivatives with RecA dramatically increased their extent of cleavage. These observations suggest that the UmuD monomer is a better substrate for the RecA-mediated cleavage reaction than the dimer.

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