J. Bacteriol., Dec 1996, 7322-7325, Vol 178, No. 24
Copyright © 1996, American Society for Microbiology

Anaerobic production of alginate by Pseudomonas aeruginosa: alginate restricts diffusion of oxygen

DJ Hassett
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Ohio 45257-0524, USA. hassetdj@ucbeh.san.uc.edu

Pseudomonas aeruginosa produced alginate and elevated algD (encoding GDPmannose 6-dehydrogenase) transcription under strict anaerobic conditions, especially when using nitrate as a terminal electron acceptor. Purified alginate added to bacterial suspensions caused a decrease in growth, suggesting that alginate contributes to oxygen limitation for the organism and likely for patients afflicted with the inherited autosomal disease cystic fibrosis.

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