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J. Bacteriol., 12 1996, 7326-7328, Vol 178, No. 24
Copyright © 1996, American Society for Microbiology

Rapid identification and cloning of bacterial transferrin and lactoferrin receptor protein genes

JA Ogunnariwo and AB Schryvers
Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.

The sequences of genes encoding the transferrin and lactoferrin receptor proteins from several bacterial species were analyzed for areas of identity in the predicted protein sequences. Degenerate oligonucleotide primers were designed and tested for their ability to amplify portions of the receptor genes. Primer pairs capable of amplifying products of the tbpA/lbpA or tbpB/lbpB genes from all species possessing these receptors were identified.


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