J. Bacteriol., 02 1996, 638-646, Vol 178, No. 3
Copyright © 1996, American Society for Microbiology

Transcription of the glutamyl-tRNA reductase (hemA) gene in Salmonella typhimurium and Escherichia coli: role of the hemA P1 promoter and the arcA gene product

P Choi, L Wang, CD Archer and T Elliott
Department of Microbiology and Immunology, West Virginia University, Health Sciences Center, Morgantown 26506, USA.

In Salmonella typhimurium and Escherichia coli, the hemA gene encodes the enzyme glutamyl-tRNA reductase, which catalyzes the first committed step in the heme biosynthetic pathway. It has recently been reported that a lac operon fusion to the hemA promoter of E. coli is induced 20- fold after starvation for heme. Induction was dependent on the transcriptional regulator ArcA, with a second transcriptional regulator, FNR, playing a negative role specifically under anaerobic conditions (S. Darie and R. P. Gunsalus, J. Bacteriol. 176:5270-5276, 1994). We have investigated the generality of this effect by examining the response to heme starvation of a number of lac operon fusions to the hemA promoters of both E. coli and S. typhimurium. We confirmed that such fusions are induced during starvation of a hemA auxotroph, but the level of induction observed was maximally sixfold and for S. typhimurium fusions it was only two- to fourfold. Sequences required for high-level expression of hemA lie within 129 bp upstream of the major (P1) promoter transcriptional start site. Mutants defective in the P1 promoter had greatly reduced hemA-lac expression both in the presence and in the absence of ALA. Mutations in arcA had no effect on hemA-lac expression in E. coli during normal growth, although the increase in expression during starvation for ALA was half that seen in an arcA+ strain. Overexpression of the arcA gene had no effect on hemA- lac expression. Primer extension analysis showed that RNA 5' ends mapping to the hemA P1 and P2 promoters were not expressed at significantly higher levels in induced cultures. These results differ from those previously reported.

This article has been cited by other articles:

• Macvanin, M., Ballagi, A., Hughes, D. (2004). Fusidic Acid-Resistant Mutants of Salmonella enterica Serovar Typhimurium Have Low Levels of Heme and a Reduced Rate of Respiration and Are Sensitive to Oxidative Stress. Antimicrob. Agents Chemother. 48: 3877-3883 [Abstract] [Full Text]  
• Elgrably-Weiss, M., Park, S., Schlosser-Silverman, E., Rosenshine, I., Imlay, J., Altuvia, S. (2002). A Salmonella enterica Serovar Typhimurium hemA Mutant Is Highly Susceptible to Oxidative DNA Damage. J. Bacteriol. 184: 3774-3784 [Abstract] [Full Text]  
• Wang, L., Elliott, M., Elliott, T. (1999). Conditional Stability of the HemA Protein (Glutamyl-tRNA Reductase) Regulates Heme Biosynthesis in Salmonella typhimurium. J. Bacteriol. 181: 1211-1219 [Abstract] [Full Text]