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J. Bacteriol., 02 1996, 911-913, Vol 178, No. 3
Copyright © 1996, American Society for Microbiology

Soft metal thiol chemistry is not involved in the transport of arsenite by the Ars pump

Y Chen, S Dey and BP Rosen
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.

The single cysteine in the ArsB protein subunit of the arsenite resistance pump was changed to serine and alanine residues. Resistance in cells expressing the two mutant arsB genes was the same as in the wild type, and the serine substitution had no effect on the arsenite transport properties. These results eliminate possible thiol chemistry in translocation. Thus, the pump uses soft metal chemistry for metalloactivation and nonmetal chemistry for oxyanion transport.

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