J. Bacteriol., 02 1996, 936-939, Vol 178, No. 3
Copyright © 1996, American Society for Microbiology

Modification of the fatty acid composition of Escherichia coli by coexpression of a plant acyl-acyl carrier protein desaturase and ferredoxin

EB Cahoon, LA Mills and J Shanklin
Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.

Expression of a plant delta 6-palmitoyl (16:0)-acyl carrier protein desaturase in Escherichia coli resulted in the accumulation of the novel monounsaturated fatty acids delta 6-hexadecenoic acid (16:1 delta 6) and delta 8-octadecenoic acid. Amounts of 16:1 delta 6 accumulated by E. coli were increased more than twofold by the expression of a plant ferredoxin together with the delta 6-16:0-acyl carrier protein desaturase.

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