J. Bacteriol., Feb 1996, 940-942, Vol 178, No. 3
Copyright © 1996, American Society for Microbiology

Isolation and characterization of a mutation that alters the substrate specificity of the Escherichia coli glucose permease

GS Begley, KA Warner, JC Arents, PW Postma and GR Jacobson
Department of Biology, Boston University, Massachusetts 02215, USA.

We isolated 10 mannitol-positive mutants from a mannitol-negative Escherichia coli strain. These mutations mapped within ptsG, encoding the glucose permease (EIIGlc), and resulted in a G-320-to-V substitution that allows EIIGlc to transport mannitol. Gly-320 lies within a putative transmembrane helix of EIIGlc that may be involved in substrate recognition.

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