J. Bacteriol., Feb 1996, 1197-1199, Vol 178, No. 4
Copyright © 1996, American Society for Microbiology

Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria

V Pelicic, JM Reyrat and B Gicquel
Unite de Genetique Mycobacterienne, Centre National de la Recherche Scientifique URA 1300, Institut Pasteur, Paris, France. vpelicic@pasteur.fr

Expression in mycobacteria of the structural gene sacB, which encodes the Bacillus subtilis levansucrase, was investigated. sacB expression is lethal to Mycobacterium smegmatis and Mycobacterium bovis BCG in the presence of 10% sucrose. sacB could thus be used as a counterselectable marker in mycobacteria.

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