J. Bacteriol., 02 1996, 1207-1212, Vol 178, No. 4
Copyright © 1996, American Society for Microbiology

VirE1 protein mediates export of the single-stranded DNA-binding protein VirE2 from Agrobacterium tumefaciens into plant cells

C Sundberg, L Meek, K Carroll, A Das and W Ream
Program in Molecular Biology, Oregon State University, Corvallis 97331, USA.

Agrobacterium tumefaciens transfers single-stranded DNAs (T strands) into plant cells. VirE1 and VirE2, which is a single-stranded DNA binding protein, are important for tumorigenesis. We show that T strands and VirE2 can enter plant cells independently and that export of VirE2, but not of T strands, depends on VirE1.

This article has been cited by other articles:

• Pattis, I., Weiss, E., Laugks, R., Haas, R., Fischer, W. (2007). The Helicobacter pylori CagF protein is a type IV secretion chaperone-like molecule that binds close to the C-terminal secretion signal of the CagA effector protein. Microbiology 153: 2896-2909 [Abstract] [Full Text]  
• Frenkiel-Krispin, D., Wolf, S. G., Albeck, S., Unger, T., Peleg, Y., Jacobovitch, J., Michael, Y., Daube, S., Sharon, M., Robinson, C. V., Svergun, D. I., Fass, D., Tzfira, T., Elbaum, M. (2007). Plant Transformation by Agrobacterium tumefaciens: MODULATION OF SINGLE-STRANDED DNA-VirE2 COMPLEX ASSEMBLY BY VirE1. J. Biol. Chem. 282: 3458-3464 [Abstract] [Full Text]  
• Hodges, L. D., Cuperus, J., Ream, W. (2004). Agrobacterium rhizogenes GALLS Protein Substitutes for Agrobacterium tumefaciens Single-Stranded DNA-Binding Protein VirE2. J. Bacteriol. 186: 3065-3077 [Abstract] [Full Text]  
• Tzfira, T., Citovsky, V. (2003). The Agrobacterium-Plant Cell Interaction. Taking Biology Lessons from a Bug. Plant Physiol. 133: 943-947 [Full Text]  
• Lee, H., Humann, J. L., Pitrak, J. S., Cuperus, J. T., Parks, T. D., Whistler, C. A., Mok, M. C., Ream, L. W. (2003). Translation Start Sequences Affect the Efficiency of Silencing of Agrobacterium tumefaciens T-DNA Oncogenes. Plant Physiol. 133: 966-977 [Abstract] [Full Text]  
• Vergunst, A. C., van Lier, M. C.M., den Dulk-Ras, A., Hooykaas, P. J.J. (2003). Recognition of the Agrobacterium tumefaciens VirE2 Translocation Signal by the VirB/D4 Transport System Does Not Require VirE1. Plant Physiol. 133: 978-988 [Abstract] [Full Text]  
• Gelvin, S. B. (2003). Agrobacterium-Mediated Plant Transformation: the Biology behind the "Gene-Jockeying" Tool. Microbiol. Mol. Biol. Rev. 67: 16-37 [Abstract] [Full Text]  
• Lee, V. T., Schneewind, O. (2001). Protein secretion and the pathogenesis of bacterial infections. Genes Dev. 15: 1725-1752 [Full Text]  
• Ward, D. V., Zambryski, P. C. (2001). The six functions of Agrobacterium VirE2. Proc. Natl. Acad. Sci. USA 98: 385-386 [Full Text]  
• Dumas, F., Duckely, M., Pelczar, P., Van Gelder, P., Hohn, B. (2001). An Agrobacterium VirE2 channel for transferred-DNA transport into plant cells. Proc. Natl. Acad. Sci. USA 10.1073/pnas.011477898v1 [Abstract] [Full Text]  
• Zhu, J., Oger, P. M., Schrammeijer, B., Hooykaas, P. J. J., Farrand, S. K., Winans, S. C. (2000). The Bases of Crown Gall Tumorigenesis. J. Bacteriol. 182: 3885-3895 [Full Text]  
• Llosa, M., Zupan, J., Baron, C., Zambryski, P. (2000). The N- and C-Terminal Portions of the Agrobacterium VirB1 Protein Independently Enhance Tumorigenesis. J. Bacteriol. 182: 3437-3445 [Abstract] [Full Text]  
• Chen, L., Li, C. M., Nester, E. W. (2000). Transferred DNA (T-DNA)-associated proteins of Agrobacterium tumefaciens are exported independently of virB. Proc. Natl. Acad. Sci. USA 10.1073/pnas.120156997v1 [Abstract] [Full Text]  
• Sundberg, C. D., Ream, W. (1999). The Agrobacterium tumefaciens Chaperone-Like Protein, VirE1, Interacts with VirE2 at Domains Required for Single-Stranded DNA Binding and Cooperative Interaction. J. Bacteriol. 181: 6850-6855 [Abstract] [Full Text]  
• Zhou, X.-R., Christie, P. J. (1999). Mutagenesis of the Agrobacterium VirE2 Single-Stranded DNA-Binding Protein Identifies Regions Required for Self-Association and Interaction with VirE1 and a Permissive Site for Hybrid Protein Construction. J. Bacteriol. 181: 4342-4352 [Abstract] [Full Text]  
• Lee, L.-Y., Gelvin, S. B., Kado, C. I. (1999). pSa Causes Oncogenic Suppression of Agrobacterium by Inhibiting VirE2 Protein Export. J. Bacteriol. 181: 186-196 [Abstract] [Full Text]  
• Gelvin, S. B. (1998). Agrobacterium VirE2 Proteins Can Form a Complex with T Strands in the Plant Cytoplasm. J. Bacteriol. 180: 4300-4302 [Abstract] [Full Text]  
• Stahl, L. E., Jacobs, A., Binns, A. N. (1998). The Conjugal Intermediate of Plasmid RSF1010 Inhibits Agrobacterium tumefaciens Virulence and VirB-Dependent Export of VirE2. J. Bacteriol. 180: 3933-3939 [Abstract] [Full Text]  
• Fullner, K. J. (1998). Role of Agrobacterium virB Genes in Transfer of T Complexes and RSF1010. J. Bacteriol. 180: 430-434 [Abstract] [Full Text]  
• Dumas, F., Duckely, M., Pelczar, P., Van Gelder, P., Hohn, B. (2001). An Agrobacterium VirE2 channel for transferred-DNA transport into plant cells. Proc. Natl. Acad. Sci. USA 98: 485-490 [Abstract] [Full Text]  
• Chen, L., Li, C. M., Nester, E. W. (2000). Transferred DNA (T-DNA)-associated proteins of Agrobacterium tumefaciens are exported independently of virB. Proc. Natl. Acad. Sci. USA 97: 7545-7550 [Abstract] [Full Text]