J. Bacteriol., Mar 1996, 1347-1350, Vol 178, No. 5
Copyright © 1996, American Society for Microbiology

Kinetics of pyrimidine(6-4)pyrimidone photoproduct repair in Escherichia coli

DR Koehler, J Courcelle and PC Hanawalt
Department of Biological Sciences, Stanford University, California 94305-5020, USA.

We compared the removal of pyrimidine(6-4)pyrimidone photoproducts [(6- 4) photoproducts] and cyclobutane pyrimidine dimers (CPDs) from the genome of repair-proficient Escherichia coli, using monoclonal antibodies specific for each type of lesion. We found that (6-4) photoproducts were removed at a higher rate than CPDs in the first 30 min following a moderate UV dose (40 J/m2). The difference in rates was less than that typically reported for cultured mammalian cells, in which the removal of (6-4) photoproducts is far more rapid than that of CPDs.

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