J. Bacteriol., Mar 1996, 1473-1475, Vol 178, No. 5
Copyright © 1996, American Society for Microbiology

The drug-binding activity of the multidrug-responding transcriptional regulator BmrR resides in its C-terminal domain

PN Markham, M Ahmed and AA Neyfakh
Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago 60612, USA.

Rhodamine and tetraphenylphosphonium, the substrates of the Bacillus subtilis multidrug efflux transporter Bmr, induce the expression of Bmr through direct interaction with its transcriptional activator BmrR. Here we show that the C-terminal domain of BmrR, expressed individually, binds both these compounds and therefore can be used as a model for molecular analysis of the phenomenon of multidrug recognition.

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