J. Bacteriol., 03 1996, 1766-1769, Vol 178, No. 6
Copyright © 1996, American Society for Microbiology

Topology of LcnD, a protein implicated in the transport of bacteriocins from Lactococcus lactis

CM Franke, KJ Leenhouts, AJ Haandrikman, J Kok, G Venema and K Venema
Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan, Haren, The Netherlands.

Four in-frame translational fusions to both the reporter proteins beta- galactosidase and alkaline phosphatase support a topological model of LcnD, a protein implicated in the transport of several bacteriocins from Lactococcus lactis, in which the N-terminal part is located intracellularly and one transmembrane helix spans the cytoplasmic membrane.

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