J. Bacteriol., 04 1996, 2051-2059, Vol 178, No. 7
Copyright © 1996, American Society for Microbiology

glc locus of Escherichia coli: characterization of genes encoding the subunits of glycolate oxidase and the glc regulator protein

MT Pellicer, J Badia, J Aguilar and L Baldoma
Department of Biochemistry, Faculty of Pharmacy, University of Barcelona, Spain.

The locus glc (min 64.5), associated with the glycolate utilization trait in Escherichia coli, is known to contain glcB, encoding malate synthase G, and the gene(s) needed for glycolate oxidase activity. Subcloning, sequencing, insertion mutagenesis, and expression studies showed five additional genes: glcC and in the other direction glcD, glcE, glcF, and glcG followed by glcB. The gene glcC may encode the glc regulator protein. Consistently a chloramphenicol acetyltransferase insertion mutation abolished both glycolate oxidase and malate synthase G activities. The proteins encoded from glcD and glcE displayed similarity to several flavoenzymes, the one from glcF was found to be similar to iron-sulfur proteins, and that from glcG had no significant similarity to any group of proteins. The insertional mutation by a chloramphenicol acetyltransferase cassette in either glcD, glcE, or glcF abolished glycolate oxidase activity, indicating that presumably these proteins are subunits of this enzyme. No effect on glycolate metabolism was detected by insertional mutation in glcG. Northern (RNA) blot experiments showed constitutive expression of glcC but induced expression for the structural genes and provided no evidence for a single polycistronic transcript.

This article has been cited by other articles:

• Eisenhut, M., Kahlon, S., Hasse, D., Ewald, R., Lieman-Hurwitz, J., Ogawa, T., Ruth, W., Bauwe, H., Kaplan, A., Hagemann, M. (2006). The Plant-Like C2 Glycolate Cycle and the Bacterial-Like Glycerate Pathway Cooperate in Phosphoglycolate Metabolism in Cyanobacteria. Plant Physiol. 142: 333-342 [Abstract] [Full Text]  
• Tavano, C. L., Podevels, A. M., Donohue, T. J. (2005). Identification of Genes Required for Recycling Reducing Power during Photosynthetic Growth. J. Bacteriol. 187: 5249-5258 [Abstract] [Full Text]  
• Tian, W., Arakaki, A. K., Skolnick, J. (2004). EFICAz: a comprehensive approach for accurate genome-scale enzyme function inference. Nucleic Acids Res 32: 6226-6239 [Abstract] [Full Text]  
• Bari, R., Kebeish, R., Kalamajka, R., Rademacher, T., Peterhansel, C. (2004). A glycolate dehydrogenase in the mitochondria of Arabidopsis thaliana. J Exp Bot 55: 623-630 [Abstract] [Full Text]  
• Rigali, S., Derouaux, A., Giannotta, F., Dusart, J. (2002). Subdivision of the Helix-Turn-Helix GntR Family of Bacterial Regulators in the FadR, HutC, MocR, and YtrA Subfamilies. J. Biol. Chem. 277: 12507-12515 [Abstract] [Full Text]  
• Beltrametti, F., Reniero, D., Backhaus, S., Hofer, B. (2001). Analysis of transcription of the bph locus of Burkholderia sp. strain LB400 and evidence that the ORF0 gene product acts as a regulator of the bphA1 promoter. Microbiology 147: 2169-2182 [Abstract] [Full Text]  
• Núñez, M. F., Teresa Pellicer, M., Badía, J., Aguilar, J., Baldomà, L. (2001). The gene yghK linked to the glc operon of Escherichia coli encodes a permease for glycolate that is structurally and functionally similar to L-lactate permease. Microbiology 147: 1069-1077 [Abstract] [Full Text]  
• Höner Zu Bentrup, K., Miczak, A., Swenson, D. L., Russell, D. G. (1999). Characterization of Activity and Expression of Isocitrate Lyase in Mycobacterium avium and Mycobacterium tuberculosis. J. Bacteriol. 181: 7161-7167 [Abstract] [Full Text]  
• Pellicer, M. T., Fernandez, C., Badia, J., Aguilar, J., Lin, E. C. C., Baldoma, L. (1999). Cross-induction of glc and ace Operons of Escherichia coli Attributable to Pathway Intersection. CHARACTERIZATION OF THE glc PROMOTER. J. Biol. Chem. 274: 1745-1752 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]