J. Bacteriol., Apr 1996, 2263-2271, Vol 178, No. 8
Copyright © 1996, American Society for Microbiology

Cell-associated glucans of Burkholderia solanacearum and Xanthomonas campestris pv. citri: a new family of periplasmic glucans

P Talaga, B Stahl, JM Wieruszeski, F Hillenkamp, S Tsuyumu, G Lippens and JP Bohin
Laboratoire de Chimie Biologique, Centre National de la Recherche Scientifique UMR 11, Universite des Sciences et Technologies de Lille, France.

The cell-associated glucans produced by Burkholderia solanacearum and Xanthomonas campestris pv. citri were isolated by trichloroacetic acid treatment and gel permeation chromatography. The compounds obtained were characterized by compositional analysis, matrix-assisted laser desorption ionization mass spectrometry, and high-performance anion- exchange chromatography. B. solanacearum synthesizes only a neutral cyclic glucan containing 13 glucose residues, and X. campestris pv. citri synthesizes a neutral cyclic glucan containing 16 glucose residues. The two glucans were further purified by high-performance anion-exchange chromatography. Methylation analysis revealed that these glucans are linked by 1,2-glycosidic bonds and one 1,6-glycosidic bond. Our 600-MHz homonuclear and 1H-13C heteronuclear nuclear magnetic resonance experiments revealed the presence of a single alpha-1,6- glycosidic linkage, whereas all other glucose residues are beta-1,2 linked. The presence of this single alpha-1,6 linkage, however, induces such structural constraints in these cyclic glucans that all individual glucose residues could be distinguished. The different anomeric proton signals allowed complete sequence-specific assignment of both glucans. The structural characteristics of these glucans contrast with those of the previously described osmoregulated periplasmic glucans.

This article has been cited by other articles:

• Rigano, L. A., Payette, C., Brouillard, G., Marano, M. R., Abramowicz, L., Torres, P. S., Yun, M., Castagnaro, A. P., Oirdi, M. E., Dufour, V., Malamud, F., Dow, J. M., Bouarab, K., Vojnov, A. A. (2007). Bacterial Cyclic {beta}-(1,2)-Glucan Acts in Systemic Suppression of Plant Immune Responses. Plant Cell 19: 2077-2089 [Abstract] [Full Text]  
• Roset, M. S., Ciocchini, A. E., Ugalde, R. A., Inon de Iannino, N. (2006). The Brucella abortus Cyclic {beta}-1,2-Glucan Virulence Factor Is Substituted with O-Ester-Linked Succinyl Residues.. J. Bacteriol. 188: 5003-5013 [Abstract] [Full Text]  
• Lacroix, J.-M., Lanfroy, E., Cogez, V., Lequette, Y., Bohin, A., Bohin, J.-P. (1999). The mdoC Gene of Escherichia coli Encodes a Membrane Protein That Is Required for Succinylation of Osmoregulated Periplasmic Glucans. J. Bacteriol. 181: 3626-3631 [Abstract] [Full Text]