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J. Bacteriol., 04 1996, 2420-2423, Vol 178, No. 8
Copyright © 1996, American Society for Microbiology

The insE open reading frame of IS1 is not required for formation of cointegrates

ET Freund and MM Susskind
Hedco Molecular Biology Laboratories, University of Southern California, Los Angeles 90089-1340, USA.

The role of the insE open reading frame in transposition of IS1 was reexamined by using an insE nonsense mutation that does not alter the amino acid sequence of InsA inhibitor or InsAB transposase. The mutant was active in all strains tested, showing that insE is not essential for formation of cointegrates.




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Copyright © 1996 by the American Society for Microbiology. All rights reserved.